农业科学与技术：英文版
農業科學與技術：英文版
농업과학여기술：영문판
Agricultural Science ＆ Technology
2012年
8期
1713-1715,1721
,共4页
小菜蛾%小油菜苗%人工饲养%毒力测定
小菜蛾%小油菜苗%人工飼養%毒力測定
소채아%소유채묘%인공사양%독력측정
Plutella xylostella L.%Brassica chinensis seedlings%Artificial rearing%Toxicity testing
[目的]优化小菜蛾（Plutella xylostella L．）的人工饲养方法，筛选防治小菜蛾的有效药剂。[方法]在温度25±1℃、相对湿度60％～75％、光照周期L：D=（14：10）h条件下，用吹塑纸收集小菜蛾卵，用小油菜（Brassica chinensis）苗饲养幼虫；同时采用浸叶法，测定小菜蛾对5种供试药剂的敏感性。[结果]用小油菜苗饲养的小菜蛾发育状况良好，其蛹重为5．4mg／头，羽化率为94．O％，每雌产卵量为135．1粒。200g／L氯虫苯甲酰胺SC、20％氟虫双酰胺WG、150g，L茚虫威EC、200g／L虫酰肼Sc和1．8％阿维菌素EC对小菜蛾3龄幼虫的LC50值分别为0．152、0．223、1．151、93．340和0．128mg／L。[结论]该饲养方法操作简单，实用性强，适合于小菜蛾的批量繁殖；阿维菌素、氯虫苯甲酰胺和氟虫双酰胺对小菜蛾有较高杀虫活性，3种农药可交替轮换使用防治小菜蛾。
[目的]優化小菜蛾（Plutella xylostella L．）的人工飼養方法，篩選防治小菜蛾的有效藥劑。[方法]在溫度25±1℃、相對濕度60％～75％、光照週期L：D=（14：10）h條件下，用吹塑紙收集小菜蛾卵，用小油菜（Brassica chinensis）苗飼養幼蟲；同時採用浸葉法，測定小菜蛾對5種供試藥劑的敏感性。[結果]用小油菜苗飼養的小菜蛾髮育狀況良好，其蛹重為5．4mg／頭，羽化率為94．O％，每雌產卵量為135．1粒。200g／L氯蟲苯甲酰胺SC、20％氟蟲雙酰胺WG、150g，L茚蟲威EC、200g／L蟲酰肼Sc和1．8％阿維菌素EC對小菜蛾3齡幼蟲的LC50值分彆為0．152、0．223、1．151、93．340和0．128mg／L。[結論]該飼養方法操作簡單，實用性彊，適閤于小菜蛾的批量繁殖；阿維菌素、氯蟲苯甲酰胺和氟蟲雙酰胺對小菜蛾有較高殺蟲活性，3種農藥可交替輪換使用防治小菜蛾。
[목적]우화소채아（Plutella xylostella L．）적인공사양방법，사선방치소채아적유효약제。[방법]재온도25±1℃、상대습도60％～75％、광조주기L：D=（14：10）h조건하，용취소지수집소채아란，용소유채（Brassica chinensis）묘사양유충；동시채용침협법，측정소채아대5충공시약제적민감성。[결과]용소유채묘사양적소채아발육상황량호，기용중위5．4mg／두，우화솔위94．O％，매자산란량위135．1립。200g／L록충분갑선알SC、20％불충쌍선알WG、150g，L인충위EC、200g／L충선정Sc화1．8％아유균소EC대소채아3령유충적LC50치분별위0．152、0．223、1．151、93．340화0．128mg／L。[결론]해사양방법조작간단，실용성강，괄합우소채아적비량번식；아유균소、록충분갑선알화불충쌍선알대소채아유교고살충활성，3충농약가교체륜환사용방치소채아。
[Objective] The research aimed to optimize laboratory artificial rearing methods for Plutella xylostella L. and to select out effective insecticides against this pest species. [Method] Brassica chinensis seedlings were cultivated in the laboratory at （25±1）℃, 60-75% RH, and a photoperiod of 14：10 （L：D） h. The green blow molding papers were used to collect eggs, and Brassica oleracea seedlings were cultivated to breed Plutella xylostella L. until the insects were ready for use. Mean-while, the susceptibility of 3rd instar larvae of Plutella xylostella L. to five types of insecticides was tested by leaf-dipping method. [Result] Plutella xylostella L. larvae were found well-grown and uniform in developmental stages with average pupa weight of 5.4 mg, pupa emergence percentage of 94.0 % and egg number of 135.1 per female. The LCso values of 3rd instar Plutella xylostella L. larvae against 200 g/L chlorantraniliprole SC, 20% flubendiamide WG, 150 g/L indoxacarb EC, 200 g/L tebufenozide SC and 1.8% abamectin EC, were 0.152, 0.223, 1.151, 93.340 and 0.128 mg/L, respectively. [Conclusion] This rearing method is easy to be implemented and of great practicality, suitable for mass propagation of P. xylostella L. Abamectin, chlorantraniliprole and flubendiamide showed high insecticidal activities, so these three insecticides can be used alternately for the field prevention and control of Plutella xylostella L.
