CAJ | 학술논문

[目的]利用SSR分子标记技术研究紫菜薹资源的亲缘关系。[方法]用从大白菜引物中筛选出的65对引物，对收集到的45份紫菜墓地方资源进行SSR分析，并对结果进行聚类分析。[结果]试验共筛选出23对SSR引物；聚类分析表明，紫菜薹亲缘关系表现出较为明显的地域性，大致可分为四川、湖南、湖北3个类群；同时，45份资源的相似系数都大于0．5（介于0．5470-0．9107之间），表明紫菜薹各品种间亲缘关系较近，遗传基础较为狭窄。[结论]该研究为紫菜薹地方品种资源的鉴定、保护以及育种利用提供了理论和技术依据。
[목적]이용SSR분자표기기술연구자채대자원적친연관계。[방법]용종대백채인물중사선출적65대인물，대수집도적45빈자채묘지방자원진행SSR분석，병대결과진행취류분석。[결과]시험공사선출23대SSR인물；취류분석표명，자채대친연관계표현출교위명현적지역성，대치가분위사천、호남、호북3개류군；동시，45빈자원적상사계수도대우0．5（개우0．5470-0．9107지간），표명자채대각품충간친연관계교근，유전기출교위협착。[결론]해연구위자채대지방품충자원적감정、보호이급육충이용제공료이론화기술의거。
[Objective] This study aimed to explore the genetic relationship of purple tsai-tai germplasms using SSR molecular markers. [Method] SSR analysis of 45 purple tsai-tai samples was conducted with 65 pairs of primers selected from cabbage primers, and the cluster analysis was carried out. [Result] A total of 23 pairs of SSR primers were screened; cluster analysis showed that the genetic relationship of purple tsai-tai germplasms had relatively significantly locality, and the 45 purple tsaitai samples can be divided into three groups of Sichuan, Hunan and Hubei; furthermore, the similarity coefficients of 45 purple tsai-tai samples were all greater than 0.5 （ranging from 0.547 0 to 0.910 7）, indicating that the closer the genetic relationship among purple tsai-tai samples is, the narrower the genetic basis will be. [Conclusion] This study provides a theoretical and technical basis for the identification, protection and breeding of the resources of local varieties of seaweed sprouts. This study provided theoretical and technical basis for the identification, protection, breeding and utilization of local purple tsai-tai resource.