CAJ | 학술논문

[目的]研究N＋离子束注入对生防菌枯草芽孢杆菌存活率和突变率的影响。[方法]对离子束注入前样品处理方法进行优化，包括枯草芽孢杆菌液体培养时间、稀释浓度、稀释溶剂和菌膜干燥时间；采用N＋离子柬注入进行诱变处理，并通过平板对峙法和牛津杯法筛选菌株。[结果]N＋离子束注入样品前处理最佳条件为：枯草芽孢杆菌液体培养20～24h，利用无菌水将菌液稀释到10^6个／ml，菌膜干燥时间为o～60min；离子束注入诱变的最佳条件为：注入能量30kev，剂量为2．0×10^14～4．0×10^14ions／cm2,枯草芽孢杆菌存活率为8．43％～26．71％，突变率为3．50％～5．43％。[结论]该研究结果为枯草芽孢杆菌离子束注入诱变育种方法的研究提供了参考依据，同时也为其他生防菌的辐射诱变育种奠定了基础。
[목적]연구N＋리자속주입대생방균고초아포간균존활솔화돌변솔적영향。[방법]대리자속주입전양품처리방법진행우화，포괄고초아포간균액체배양시간、희석농도、희석용제화균막간조시간；채용N＋리자간주입진행유변처리，병통과평판대치법화우진배법사선균주。[결과]N＋리자속주입양품전처리최가조건위：고초아포간균액체배양20～24h，이용무균수장균액희석도10^6개／ml，균막간조시간위o～60min；리자속주입유변적최가조건위：주입능량30kev，제량위2．0×10^14～4．0×10^14ions／cm2,고초아포간균존활솔위8．43％～26．71％，돌변솔위3．50％～5．43％。[결론]해연구결과위고초아포간균리자속주입유변육충방법적연구제공료삼고의거，동시야위기타생방균적복사유변육충전정료기출。
[Objective] This study was to investigate the effect of N＋ ion beam implantation on the survival rate and mutation rate of biocontrol strain Bacillus subtilis. [Method] The factors influencing B. subtilis ion beam implantation, including culture time, dilution concentration, solvent, drying time of mycoderm were optimized. B. subtilis cells were implanted by using ion beam at dose of 2.0×10^14～4.0×10^14 ions/cm2 and the energy of 30 kev. Then the methods of culturing colonies confronting each other on plate and Oxford cup diffusion were used to screening strains. [Result] The optimal parameters were found as follows： culture in liquid for 20-24 h, dilution with sterile water to 106 cells/ml and drying time of 60 min for sample preparation; the optimal N＋ ion beam implantation dose of 2.0×10^14～4.0×10^14 ions/cm2 at the energy of 30 kev, the survival rate of 8.43%-26.71% and the mutation rate of 3.50%-5.43%. [Conclusion] This study provided reference for ion beam implantation mutation of B. subtilis.