CAJ | 학술논문

[目的]研究灵芝群体交配基因型，并与酯酶同工酶试验相比较，探讨它们在群体亲缘关系分析上的异同点。[方法]采用OWE—SOJ技术对24个灵芝菌株的单孢分离菌株进行标准交配型鉴定，及群体间交配基因型的测定，并结合酯酶同工酶试验对群体间的亲缘关系进行分析。[结果]鉴定出7大类交配基因型，并发现A因子含有4个等位基因，B因子含有4个等位基因。及一个特殊的A混合基因，四类交配型出现了一定程度的偏分离。酯酶同工酶试验检测出了28条不同酯酶酶谱带，在相似系数为0．73214时，样品被分为9大类。比较交配基因型分析与酯酶同工酶试验结果，发现两者具有很高的相似性。[结论]交配基因型测定可以作为分析菌株间差异和鉴定品种的一种重要补充手段。
[목적]연구령지군체교배기인형，병여지매동공매시험상비교，탐토타문재군체친연관계분석상적이동점。[방법]채용OWE—SOJ기술대24개령지균주적단포분리균주진행표준교배형감정，급군체간교배기인형적측정，병결합지매동공매시험대군체간적친연관계진행분석。[결과]감정출7대류교배기인형，병발현A인자함유4개등위기인，B인자함유4개등위기인。급일개특수적A혼합기인，사류교배형출현료일정정도적편분리。지매동공매시험검측출료28조불동지매매보대，재상사계수위0．73214시，양품피분위9대류。비교교배기인형분석여지매동공매시험결과，발현량자구유흔고적상사성。[결론]교배기인형측정가이작위분석균주간차이화감정품충적일충중요보충수단。
[Objective] The mating genotype was studied and compared with esterase isozyme of Ganoderma lucidum populations between groups in order to clarify their differences in genetic relationship analysis. [Method] OWE-SOJ technique was applied to identify standard mating types and determinate mating genotype between groups of monokaryons isolates from 24 G. lucidum stains. Genetic relationships were analyzed by combined group mating genotype determination with esterase isozyme assay. [Result] All strains of G. lucidum could be divided into 7 large groups of the mating genotype. Four alleles of A factor, four alleles of B factor and one mixed alleles of A factor were found in this study. Distorted segregation ratio among monokaryon mycelia of G. lucidum had been observed in four kinds of mat- ing types to some extent. Twenty-eight different types of enzyme bands were determined in esterase isozyme test, Twenty-four strains of G. lucidum could be divided into 9 large groups through the cluster analysis when the genetic similarity coefficient was 0.73214. Comparing the results of mating genotype analysis and esterase isozyme analysis, it showed great similarity. [Conclusion] Mating genotype analysis could be used as an important supplementary method for strain identification and genetic diversity research.