农业科学与技术:英文版
農業科學與技術:英文版
농업과학여기술:영문판
Agricultural Science & Technology
2012年
8期
1645-1650,1753
,共7页
马亭亭%周宜君%高飞%余丽%刘冉%刘楠%隋欣
馬亭亭%週宜君%高飛%餘麗%劉冉%劉楠%隋訢
마정정%주의군%고비%여려%류염%류남%수흔
拟南芥%AtPERs%生物信息学分析
擬南芥%AtPERs%生物信息學分析
의남개%AtPERs%생물신식학분석
Arabidopsis%AtPERs%Bioinformatical analysis
植物过氧化物酶(POD)属于多基因家族,不仅是植物体内清除活性氧自由基的重要酶类之一,而且参与多种生理生化过程,在维系植物生长发育过程中发挥重要的作用。采用生物信息学方法对拟南芥过氧化物酶家族的73个基因编码的蛋白质序列的结构和功能进行了分析,其中包含氨基酸的数量、等电点、跨膜结构域、信号肽、二级结构组成及磷酸化位点等,并用Mega4.0软件构建了去除信号肽前后的系统进化树,旨在了解其结构特征。对已经进行功能研究的成员进行结构分析,以此来揭示结构与功能之间的联系,为植物抵御氧化胁迫方面研究提供理论基础。
植物過氧化物酶(POD)屬于多基因傢族,不僅是植物體內清除活性氧自由基的重要酶類之一,而且參與多種生理生化過程,在維繫植物生長髮育過程中髮揮重要的作用。採用生物信息學方法對擬南芥過氧化物酶傢族的73箇基因編碼的蛋白質序列的結構和功能進行瞭分析,其中包含氨基痠的數量、等電點、跨膜結構域、信號肽、二級結構組成及燐痠化位點等,併用Mega4.0軟件構建瞭去除信號肽前後的繫統進化樹,旨在瞭解其結構特徵。對已經進行功能研究的成員進行結構分析,以此來揭示結構與功能之間的聯繫,為植物牴禦氧化脅迫方麵研究提供理論基礎。
식물과양화물매(POD)속우다기인가족,불부시식물체내청제활성양자유기적중요매류지일,이차삼여다충생리생화과정,재유계식물생장발육과정중발휘중요적작용。채용생물신식학방법대의남개과양화물매가족적73개기인편마적단백질서렬적결구화공능진행료분석,기중포함안기산적수량、등전점、과막결구역、신호태、이급결구조성급린산화위점등,병용Mega4.0연건구건료거제신호태전후적계통진화수,지재료해기결구특정。대이경진행공능연구적성원진행결구분석,이차래게시결구여공능지간적련계,위식물저어양화협박방면연구제공이론기출。
Plant peroxidase (POD) belongs to multigene family, which not is only one of the important enzymes responsible for the removal of active oxygen radicals, but also participates in a variety of physiological and biochemical processes and plays a crucial role in the maintenance of plant growth and development. In this study, the structures and functions of proteins encoded by 73 gene of POD family in Arabidopsis were analyzed with bioinformatics method, including the number of amino acids, isoelectric point, transmemberane domains, signal peptides, secondary structures and phosphorylation sites, and the phylogenic trees with and without signal peptides were constructed by using Mega4.0 software, to investigate the structural characteristics. In addition, the structures of AtPER members were analyzed, to reveal the relationship between the structures and functions, thereby providing theoretical basis for the research of plant oxidative stress resistance.