农业科学与技术:英文版
農業科學與技術:英文版
농업과학여기술:영문판
Agricultural Science & Technology
2012年
8期
1628-1630,1636
,共4页
许鹏辉%邢顺林%段双全%张正果
許鵬輝%邢順林%段雙全%張正果
허붕휘%형순림%단쌍전%장정과
寄生%西藏冬虫夏草%镰孢属真菌%孢子形态
寄生%西藏鼕蟲夏草%鐮孢屬真菌%孢子形態
기생%서장동충하초%렴포속진균%포자형태
Parasitism%Tibetan Cordyceps sinensis,Fusadum fungi%Spore morphology
[目的]对西藏冬虫夏草寄生镰孢属真菌进行生物学特性分析。[方法]以从新鲜冬虫夏草体上分离得到了镰刀菌菌株为材料,对其进行菌株和孢子形态观察。[结果]此种菌株在大米培养基上生长茂盛,菌丝呈白色绒毛状,基质呈米黄色到粉红色;菌株在(18±4)℃条件下培养2周后,产生乳白色突起,里面有淡黄色的厚垣孢子,分大小2种孢子;通过安斯沃斯分类系统查阅分离到的菌株属于丝孢纲瘤座孢目镰孢属的一种;对菌株的大孢子观察发现有3种类型大孢子,而其中2种的形态比较特别,在安斯沃斯分类系统中未有描述。[结论]此次从两藏冬虫夏草上分离到的菌株,与查阅相关资料记载的镰孢属真菌结构有很大区别,有待于做进一步研究。
[目的]對西藏鼕蟲夏草寄生鐮孢屬真菌進行生物學特性分析。[方法]以從新鮮鼕蟲夏草體上分離得到瞭鐮刀菌菌株為材料,對其進行菌株和孢子形態觀察。[結果]此種菌株在大米培養基上生長茂盛,菌絲呈白色絨毛狀,基質呈米黃色到粉紅色;菌株在(18±4)℃條件下培養2週後,產生乳白色突起,裏麵有淡黃色的厚垣孢子,分大小2種孢子;通過安斯沃斯分類繫統查閱分離到的菌株屬于絲孢綱瘤座孢目鐮孢屬的一種;對菌株的大孢子觀察髮現有3種類型大孢子,而其中2種的形態比較特彆,在安斯沃斯分類繫統中未有描述。[結論]此次從兩藏鼕蟲夏草上分離到的菌株,與查閱相關資料記載的鐮孢屬真菌結構有很大區彆,有待于做進一步研究。
[목적]대서장동충하초기생렴포속진균진행생물학특성분석。[방법]이종신선동충하초체상분리득도료렴도균균주위재료,대기진행균주화포자형태관찰。[결과]차충균주재대미배양기상생장무성,균사정백색융모상,기질정미황색도분홍색;균주재(18±4)℃조건하배양2주후,산생유백색돌기,리면유담황색적후원포자,분대소2충포자;통과안사옥사분류계통사열분리도적균주속우사포강류좌포목렴포속적일충;대균주적대포자관찰발현유3충류형대포자,이기중2충적형태비교특별,재안사옥사분류계통중미유묘술。[결론]차차종량장동충하초상분리도적균주,여사열상관자료기재적렴포속진균결구유흔대구별,유대우주진일보연구。
[Objective] This study aimed to investigate the biological characteristics of parasitic Fusarium fungi from tibetan Cordyceps sinensis. [Method] A Fusarium strain was isolated from fresh tibetan Cordyceps sinensis as the experimental material, to observe the morphologies of Fusarium mycelium and spores. [Result] The isolated Fusarium fungus strain grew lushly on rice medium, with white and villiform myceliurn, and the villous stroma appeared in colors from beige to pink. After cultivated at (18 ± 4) ℃ for 14 d, the Fusarium strain produced milky white protrusions containing pale yellow chlamydospores, which can be divided into microconidiums and macroconidiums based on the different sizes. According to Ainsworth's classification system, the isolated strain belongs to Fusarium, Tuberculariales, Hyphomycetes, adelomycete subphylum. Further observation identified three types of macroconidium, and two of them showed distinct morphology, which was not described in Ainsworth's classification system. [Conclusion] The structure of the strain isolated from tibetan Cordyceps sinensis is significantly different from that of Fusariurn fungi recorded in relevant literatures, which requires further investigation.