科技创新导报
科技創新導報
과기창신도보
SCIENCE AND TECHNOLOGY CONSULTING HERALD
2012年
22期
4-5,8
,共3页
里氏木霉%绿色木霉%康氏木霉%氮源%碳源%发酵条件%酶活
裏氏木黴%綠色木黴%康氏木黴%氮源%碳源%髮酵條件%酶活
리씨목매%록색목매%강씨목매%담원%탄원%발효조건%매활
Trichoderma reesei%trichoderma viridestrichoderma koningii~Nitrogen source:Carbon source%Fermentation conditionsEnzyme live
以里氏木霉、绿色木霉及康氏木霉作菌种,分别用微晶纤维素、玉米芯粉、稻草粉、麸皮作碳源,用豆饼粉、蛋白胨、硫酸胺、硝酸胺、尿素作氮源进行摇瓶发酵培养,并对其摇瓶发酵条件进行了研究,结果显示：不同菌种对碳氮源的要求不同,三种木霉培养基的最佳组成分别为：里氏木霉,微晶纤维素1%,磷酸二氢钾0.1%,硝酸铵0.3%,微量元素液0.1%,吐温-801000ppm,装量70ml/500mlΔ;康氏木霉,微晶纤维素1%,磷酸二氢钾0.1%,硫酸铵0.5%,微量元素液0.1%,吐温-80 1500ppm,装量50ml/500mlΔ;绿色木霉,粗玉米芯粉4.5%,豆饼粉4.0%,麸皮0.5%,蛋白胨0.1%,kH2PO40.1%,（NH4）2SO40.1%,MgSO40.05%,装量50ml/500mlΔ。培养温度均为28±1℃。
以裏氏木黴、綠色木黴及康氏木黴作菌種,分彆用微晶纖維素、玉米芯粉、稻草粉、麩皮作碳源,用豆餅粉、蛋白胨、硫痠胺、硝痠胺、尿素作氮源進行搖瓶髮酵培養,併對其搖瓶髮酵條件進行瞭研究,結果顯示：不同菌種對碳氮源的要求不同,三種木黴培養基的最佳組成分彆為：裏氏木黴,微晶纖維素1%,燐痠二氫鉀0.1%,硝痠銨0.3%,微量元素液0.1%,吐溫-801000ppm,裝量70ml/500mlΔ;康氏木黴,微晶纖維素1%,燐痠二氫鉀0.1%,硫痠銨0.5%,微量元素液0.1%,吐溫-80 1500ppm,裝量50ml/500mlΔ;綠色木黴,粗玉米芯粉4.5%,豆餅粉4.0%,麩皮0.5%,蛋白胨0.1%,kH2PO40.1%,（NH4）2SO40.1%,MgSO40.05%,裝量50ml/500mlΔ。培養溫度均為28±1℃。
이리씨목매、록색목매급강씨목매작균충,분별용미정섬유소、옥미심분、도초분、부피작탄원,용두병분、단백동、류산알、초산알、뇨소작담원진행요병발효배양,병대기요병발효조건진행료연구,결과현시：불동균충대탄담원적요구불동,삼충목매배양기적최가조성분별위：리씨목매,미정섬유소1%,린산이경갑0.1%,초산안0.3%,미량원소액0.1%,토온-801000ppm,장량70ml/500mlΔ;강씨목매,미정섬유소1%,린산이경갑0.1%,류산안0.5%,미량원소액0.1%,토온-80 1500ppm,장량50ml/500mlΔ;록색목매,조옥미심분4.5%,두병분4.0%,부피0.5%,단백동0.1%,kH2PO40.1%,（NH4）2SO40.1%,MgSO40.05%,장량50ml/500mlΔ。배양온도균위28±1℃。
the measuring trichoderma viride and green wooden mold and mildew for KangShiMu strains,respectively for microcrystalline cellulose,several powder,rice straw powder,bran for carbon sources,with DouBing powder,specially designed,acid amine,nitric acid amine,urea nitrogen source for wave bottle fermentation cultivation,and the wave bottle fermentation conditions were studied,and the results show that different strains of carbon nitrogen source of different requirements,the three kinds of trichoderma viride medium of the best composition are：the magnitude trichoderma viride and microcrystalline cellulose 1%,potassium dihydrogen phosphate 0.1%,ammonium nitrate by 0.3%,trace elements liquid 0.1%,twain-80 1000PPM,pack of 70ml/500ml A IKangShiMu mildew, microcrystalline cellulose 1%, potassium dihydrogen phosphate 0. 1%, 0.5% ammonium sulfate, trace elements liquid 0.1%, twain-80 1500PPM,volume of fill 50ml/500ml △：The green wooden mold,several thick powder 4.5%,DouBing powder 4.0%,0.5% bran, specially designed by 0. l%,kH2PO40, l%,（NH4） 2 SO40. 1%,MgSO40.05%, 50ml for content/500ml A .The incubation temperature are 28 degree is obtai ℃.
