CAJ | 학술논문

目的：研究间充质干细胞向成骨细胞分化的高分辨MR波谱(MRS)代谢特征。材料与方法培养获取间胎儿脐带充质干细胞，建立体外诱导间充质干细胞向成骨细胞分化的模型，利用甲醇/氯仿获取细胞代谢提取物，经后处理后利用9.4 T高分辨率MRS检测代谢提取物频谱特征。结果诱导间充质干细胞向成骨细胞分化前后，位于2.02 ppm及2.25 ppm的脂质峰明显减低，且在1.77 ppm出现一新的代谢峰，该代谢峰可能为亮氨酸。结论间充质干细胞成骨分化后MRS具有一定特征，位于1.77 ppm的代谢峰可能做为间充质干细胞成骨分化的生物学标识。
목적：연구간충질간세포향성골세포분화적고분변MR파보(MRS)대사특정。재료여방법배양획취간태인제대충질간세포，건입체외유도간충질간세포향성골세포분화적모형，이용갑순/록방획취세포대사제취물，경후처리후이용9.4 T고분변솔MRS검측대사제취물빈보특정。결과유도간충질간세포향성골세포분화전후，위우2.02 ppm급2.25 ppm적지질봉명현감저，차재1.77 ppm출현일신적대사봉，해대사봉가능위량안산。결론간충질간세포성골분화후MRS구유일정특정，위우1.77 ppm적대사봉가능주위간충질간세포성골분화적생물학표식。
Objective: To explore the metabolite profile of mesenchymal stem cells (MSCs) underwent osteogenic differentiation using 9.4 T high resolution MR spectroscopy. Materials and Methods: Human umbilical cord mesenchymal stem cells were cultured and collected. MSCs were induced osteogenic differentiation for 2 weeks and cell metabolite extractions were prepared by methanol-chloroform (M/C) method. Cell extractions were then analyzed on a 9.4 T MRS device. Results:After osteogenic inducement, the fatty acid metabolite peak at 2.02 ppm and 2.25 ppm obviously decreased, and there appeared a new metabolite peak at about 1.77 ppm, which is most probably representative of leucine. Conclusions:There are some speciifc characteristics on MRS of MSCs underwent osteogenic differentiation. The new arised peak at 1.77 ppm may serve as the biomarker for mesenchymal stem cells osteogenic differentiation.