CAJ | 학술논문

目的：探讨骨桥蛋白对胰腺癌细胞中金属基质蛋白酶-9转录后调控的影响。方法采用荧光定量PCR、荧光素酶报告基因技术检测骨桥蛋白对胰腺癌细胞金属基质蛋白酶-9转录后调控的影响。结果骨桥蛋白促进金属基质蛋白酶-9 mRNA半衰期的延长，上调含金属基质蛋白酶-93′UTR的荧光素酶报告基因的表达。结论骨桥蛋白上调胰腺癌细胞株PANC-1中金属基质蛋白酶-9转录后调控，其主要作用位点在金属基质蛋白酶-93′UTR。
목적：탐토골교단백대이선암세포중금속기질단백매-9전록후조공적영향。방법채용형광정량PCR、형광소매보고기인기술검측골교단백대이선암세포금속기질단백매-9전록후조공적영향。결과골교단백촉진금속기질단백매-9 mRNA반쇠기적연장，상조함금속기질단백매-93′UTR적형광소매보고기인적표체。결론골교단백상조이선암세포주PANC-1중금속기질단백매-9전록후조공，기주요작용위점재금속기질단백매-93′UTR。
Objective To investigate the effects of osteopontin on post-transcriptional regulation of matrix metalloproteinase-9(MMP-9) gene expression in PANC-1 cells.Methods Real-time PCR and luciferase reporter gene assay were used to detect the effects of osteopontin on post-transcriptional regulation of MMP-9 gene expression in PANC-1 cells.Results Osteopontin prolonged the half-life of MMP-9 mRNA and up-regulated the expression of luciferase in report gene plasmid which contained 3′UTR of MMP-9 mRNA in PANC-1 cells.Conclusion Osteopontin activates the expression of MMP-9 partly through affecting post-transcriptional regulation of MMP-9 gene expression in PANC-1 cells, whose primary target is 3′UTR of MMP-9.