CAJ | 학술논문

目的：建立高效液相色谱（HPLC）法测定骨痹舒片中士的宁和马钱子碱的含量。方法：采用HPLC法测定Agilent C18色谱柱（4.6 mm×250 mm，5μm），流动相：甲醇250 g·L-1∶缓冲液（水∶乙酸∶三乙胺=230∶2.4∶0.3）750 g·L-1；体积流量：1.0 mL·min-1；检测波长254 nm；柱温25℃。结果：士的宁在0.1208～1.4496μg线性关系良好，相关系数为0.9999，士的宁平均回收率为99.01%，RSD为0.91%；马钱子碱在0.0948～1.1376μg线性关系良好，相关系数为1，马钱子碱平均回收率为97.37%， RSD为1.26%。结论：该方法快速、准确、重现性好，可用于骨痹舒片中士的宁和马钱子碱的质量控制。
목적：건립고효액상색보（HPLC）법측정골비서편중사적저화마전자감적함량。방법：채용HPLC법측정Agilent C18색보주（4.6 mm×250 mm，5μm），류동상：갑순250 g·L-1∶완충액（수∶을산∶삼을알=230∶2.4∶0.3）750 g·L-1；체적류량：1.0 mL·min-1；검측파장254 nm；주온25℃。결과：사적저재0.1208～1.4496μg선성관계량호，상관계수위0.9999，사적저평균회수솔위99.01%，RSD위0.91%；마전자감재0.0948～1.1376μg선성관계량호，상관계수위1，마전자감평균회수솔위97.37%， RSD위1.26%。결론：해방법쾌속、준학、중현성호，가용우골비서편중사적저화마전자감적질량공제。
Objective:To establish the HPLC method for determination of strychnine and brucine in gubishu tablet.Methods:By the method of HPLC,Chromatographic column Agilent C18(4.6 mm × 250 mm,5μm);the mobile phase:was 250 g·L-1 methanol:750 g·L-1 buffer solution(water:acetic acid:triethylamine=230:2.4:0.3); volume lfow :1.0 mL min-1;detected wavelength:254 nm;column temperature:25℃.Results:The linear relationship of strychnine was good in 0.1208 ~ 1.4496 μg with the correlation coefficient 0.999 9, the average recovery 99.01% and RSD 0.91%.The linear relationship of brucine in 0.0948 ~ 1.1376 μg was good,with the correlation coefifcient 1, the average recovery 97.37% and RSD 1.26%.Conclusion:This method is rapid,accurate with good reproducibility,which can be used for the quality control of strychnine and brucin in gubishu tablet.