CAJ | 학술논문

目的：建立盐酸头孢替安中聚合物的含量测定方法。方法色谱柱葡聚糖凝胶Sephadex G-10柱（300mm×10mm）；流动相 A 为0.1mol/L 磷酸盐缓冲液（pH7.0），流动相B 为水；检测波长254nm，进样量200μL，流速1.5mL/min。结果盐酸头孢替安样品溶液的质量浓度在1.04～30.24mg/mL与聚合物峰面积之间的线性关系良好（r=0.9990）。结论本方法简便、准确、灵敏度高，适用于盐酸头孢替安聚合物的控制。
목적：건립염산두포체안중취합물적함량측정방법。방법색보주포취당응효Sephadex G-10주（300mm×10mm）；류동상 A 위0.1mol/L 린산염완충액（pH7.0），류동상B 위수；검측파장254nm，진양량200μL，류속1.5mL/min。결과염산두포체안양품용액적질량농도재1.04～30.24mg/mL여취합물봉면적지간적선성관계량호（r=0.9990）。결론본방법간편、준학、령민도고，괄용우염산두포체안취합물적공제。
Objectivepurpose for cefotiam hydrochloride Anaka determination of polymer content.MethodDextran gellSephadex chromatography column G-10column (300mmx10mm),mobile phase a is 0.1 mol/llphosphate bufer(pH7), mobile phase b for water detection wavelength of 254 NM,injection volume 200μl,velocity 1.5 mL/min. Resultsfor cefotiam hydrochloride concentration of the sample solution in 1.04~30.24 peak area of the linear relationship between mg/mlland polymer(r=0.9990).Conclusion the method is simple,accurate,high sensitivity,suitable cefotiam polymer hydrochloride controled.