CAJ | 학술논문

埃博霉素B是由纤维堆囊菌产生的具有抗癌作用的大环内酯类化合物，是一种新型的抗肿瘤谱广、作用性强的抗肿瘤药物．以纤维堆囊菌SoF5-76为供试菌株，采用单因素试验和正交试验设计，对其产埃博霉素B的发酵条件进行了优化，确定了最优发酵条件为：初始pH 7．4，装液量50/250 mL ，转速200 r/min ，种龄60 h ，接种量10％，温度30℃，发酵周期6 d ．在此最优发酵条件下，埃博霉素B的产量为35．24 m g/L ，比优化前提高了17．67％．
애박매소B시유섬유퇴낭균산생적구유항암작용적대배내지류화합물，시일충신형적항종류보엄、작용성강적항종류약물．이섬유퇴낭균SoF5-76위공시균주，채용단인소시험화정교시험설계，대기산애박매소B적발효조건진행료우화，학정료최우발효조건위：초시pH 7．4，장액량50/250 mL ，전속200 r/min ，충령60 h ，접충량10％，온도30℃，발효주기6 d ．재차최우발효조건하，애박매소B적산량위35．24 m g/L ，비우화전제고료17．67％．
Epothilone B is a novel anti-cancer macrolide compound produced by sorangium cel-lulosum and show s the excellent activity against tumor both in antitumor spectra and in effect .This paper focused on the optimization of fermentation conditions of epothilone B pro-duced by sorangium cellulosum SoF5-76 .One-variable-at-a-time design and orthogonal exper-iments were employed to obtain the optimum fermentation conditons .The results were as follows :initial pH of fermentation medium 7 .4 ,medium volume 50/250 mL ,shaker revolu-tion 200 r/min ,seed age 60 h ,inoculation size 10% culture temperature 30 ℃ ,fermentation period 6 d .Under the optimized conditions ,the epothilone B production reached at 36 .57 mg/L and was 22 .1% higher than preliminary fermentation conditions .