CAJ | 학술논문

采用96孔板微量稀释法，对从水杨梅中分离纯化得到的化合物的抑菌活性进行研究，测定获得了化合物对大肠杆菌、绿脓杆菌、乳链球菌、金黄色葡萄球菌、枯草芽孢杆菌的最小抑菌浓度（MIC）．结果表明，水杨梅中得到的β-谷甾醇、熊果酸、山奈酚、槲皮素、山奈酚-3-O-β-D-葡萄糖苷、槲皮素-3-O-β-D-葡萄糖苷和胡萝卜苷对测试的5种细菌具有不同的抑制作用，其中山奈酚对大肠杆菌的最小抑菌浓度达到7．8μg/mL ．并对得到的化合物山奈酚、槲皮素、山奈酚-3-O-β-D-葡萄糖苷、槲皮素-3-O-β-D-葡萄糖苷以及从其它植物样品中提取分离到的芹菜素、木犀草素、芹菜素-7-O-β-D-葡萄糖苷、木犀草素-7-O-β-D-葡萄糖苷的抗氧化活性进行研究，以抗坏血酸和BHT 为对照，采用DPPH法测定自由基清除率，结果表明，水杨梅中分离得到的黄酮醇类化合物具有良好的抗氧化性，黄酮类化合物的抗氧化能力由强到弱顺序为槲皮素＞槲皮素-3-O-β-D-葡萄糖苷＞山奈酚＞木犀草素＞木犀草素-7-O-β-D-葡萄糖苷＞山奈酚-3-O-β-D-葡萄糖苷＞芹菜素＞芹菜素-7-O-β-D-葡萄糖苷．
채용96공판미량희석법，대종수양매중분리순화득도적화합물적억균활성진행연구，측정획득료화합물대대장간균、록농간균、유련구균、금황색포도구균、고초아포간균적최소억균농도（MIC）．결과표명，수양매중득도적β-곡치순、웅과산、산내분、곡피소、산내분-3-O-β-D-포도당감、곡피소-3-O-β-D-포도당감화호라복감대측시적5충세균구유불동적억제작용，기중산내분대대장간균적최소억균농도체도7．8μg/mL ．병대득도적화합물산내분、곡피소、산내분-3-O-β-D-포도당감、곡피소-3-O-β-D-포도당감이급종기타식물양품중제취분리도적근채소、목서초소、근채소-7-O-β-D-포도당감、목서초소-7-O-β-D-포도당감적항양화활성진행연구，이항배혈산화BHT 위대조，채용DPPH법측정자유기청제솔，결과표명，수양매중분리득도적황동순류화합물구유량호적항양화성，황동류화합물적항양화능력유강도약순서위곡피소＞곡피소-3-O-β-D-포도당감＞산내분＞목서초소＞목서초소-7-O-β-D-포도당감＞산내분-3-O-β-D-포도당감＞근채소＞근채소-7-O-β-D-포도당감．
The Minmum Inhibiting Concentration (MIC) of compounds isolated from Geum aleppicum were measured by microdilution method on 96-well plates against E .coli ,P . aeruginosa ,S .lactis ,S .aureus ,B .subtilis .The results showed that β-sitosterol ,ursolic acid , kaempferol ,quercetin ,kaempferol-3-O-β-D-glucoside ,quercetin-3-O-β-D-glucoside and daucosterol had inhibiting activity to the tested bacteria .Kaempferol had notable inhibi-ting microorganism activity to E .coli ,with the MIC 7 .8 μg/mL .The antioxidant effects of compounds kaempferol ,quercetin ,kaempferol-3-O-β-D-glucoside ,quercetin-3-O-β-D-glu-coside ,apigenin ,luteolin ,apigenin-7-O-β-D-glucoside and luteolin-7-O-β-D-glucoside were tested .The DPPH radicals-cavenging capacity was measured against various concentrations with ascorbic acid and BHT as contrasts .The result of antioxidant activity test showed that flavonols from Geum aleppicum have a good antioxidant activity .