陕西科技大学学报（自然科学版）
陝西科技大學學報（自然科學版）
협서과기대학학보（자연과학판）
JOURNAL OF SHAANXI UNIVERSITY OF SCIENCE & TECHNOLOGY
2014年
1期
114-118
,共5页
高效液相色谱法%金花菌%固态发酵%他汀类
高效液相色譜法%金花菌%固態髮酵%他汀類
고효액상색보법%금화균%고태발효%타정류
high-performance liquid chromatography%Jinhua fungi%solid-state fermentation%statins
以金花菌固态培养物为研究对象，选择降脂类物质洛伐他汀标品为对照，采用高效液相色谱法，选用色谱柱C18柱，以甲醇∶水（80∶20）为流动相，流速为1．0 mL/min ，在波长238 nm处检测金花菌固态发酵中的他汀类物质．结果显示：此方法具有良好的分离度和线性关系，精密度、重现性、准确度良好，平均回收率为99％，RSD为1．39％，最低检测限为2．0μg/m L ．外标法得出金花菌乙酸乙酯提取液中洛伐他汀含量为0．049 m g/kg ．
以金花菌固態培養物為研究對象，選擇降脂類物質洛伐他汀標品為對照，採用高效液相色譜法，選用色譜柱C18柱，以甲醇∶水（80∶20）為流動相，流速為1．0 mL/min ，在波長238 nm處檢測金花菌固態髮酵中的他汀類物質．結果顯示：此方法具有良好的分離度和線性關繫，精密度、重現性、準確度良好，平均迴收率為99％，RSD為1．39％，最低檢測限為2．0μg/m L ．外標法得齣金花菌乙痠乙酯提取液中洛伐他汀含量為0．049 m g/kg ．
이금화균고태배양물위연구대상，선택강지류물질락벌타정표품위대조，채용고효액상색보법，선용색보주C18주，이갑순∶수（80∶20）위류동상，류속위1．0 mL/min ，재파장238 nm처검측금화균고태발효중적타정류물질．결과현시：차방법구유량호적분리도화선성관계，정밀도、중현성、준학도량호，평균회수솔위99％，RSD위1．39％，최저검측한위2．0μg/m L ．외표법득출금화균을산을지제취액중락벌타정함량위0．049 m g/kg ．
The statins in solid-state fermentation of Jinhua fungi was detected by the technolo-gy of high-performance liquid chromatography ,while lovastatin for reference .The chroma-tography condition of statins in Jinhua fungi included C18 column ,using methanol :H2 O (80∶20) as mobile phase ,the flow rate was 1 .0 mL/min and the detective wavelength was set at 238 nm .The result shows that the average recovery rate of this method was 99% .The RSD (Relative Standard Deviation ) was 1 .39% .The limit was 2 .0 μg/mL .The method show s good separation degree ,linear relationship and good precision ,reproducibility ,accu-racy .The stains in Jinhua fungi ethyl acetate extracting solution was 0 .049 mg/kg which ob-tained by external standard method .
