华南农业大学学报
華南農業大學學報
화남농업대학학보
JOURNAL OF SOUTH CHINA AGRICULTURAL UNIVERSITY
2014年
1期
110-113
,共4页
张姿丽%蒋锋%刘鹏飞%陈青春%张媛%王晓明
張姿麗%蔣鋒%劉鵬飛%陳青春%張媛%王曉明
장자려%장봉%류붕비%진청춘%장원%왕효명
甜玉米%雄穗一级分枝数%复合区间作图%QTL
甜玉米%雄穗一級分枝數%複閤區間作圖%QTL
첨옥미%웅수일급분지수%복합구간작도%QTL
sweet corn%tassel primary branch number%multiple interval mapping%QTL
[目的]为改良玉米雄穗性状.[方法]以雄穗一级分枝数有显著差异的超甜玉米自交系T4和T19为亲本,构建了包含232个单株的F2群体,考察雄穗一级分枝数,利用复合区间作图法进行QTL定位.[结果和结论]结果获得一张包含77个SSR标记的遗传连锁图谱,全长868.7 cM,标记平均间距为11.28 cM,共检测到4个与超甜玉米雄穗一级分枝数相关的QTL位点,分别位于玉米第4、7、8染色体上,可解释5.08%~17.71%的表型变异.主效QTL位点qTBN-4位于第8染色体,可解释17.71%的表型变异.这些QTLs将为雄穗一级分枝数的分子标记辅助选择提供依据.
[目的]為改良玉米雄穗性狀.[方法]以雄穗一級分枝數有顯著差異的超甜玉米自交繫T4和T19為親本,構建瞭包含232箇單株的F2群體,攷察雄穗一級分枝數,利用複閤區間作圖法進行QTL定位.[結果和結論]結果穫得一張包含77箇SSR標記的遺傳連鎖圖譜,全長868.7 cM,標記平均間距為11.28 cM,共檢測到4箇與超甜玉米雄穗一級分枝數相關的QTL位點,分彆位于玉米第4、7、8染色體上,可解釋5.08%~17.71%的錶型變異.主效QTL位點qTBN-4位于第8染色體,可解釋17.71%的錶型變異.這些QTLs將為雄穗一級分枝數的分子標記輔助選擇提供依據.
[목적]위개량옥미웅수성상.[방법]이웅수일급분지수유현저차이적초첨옥미자교계T4화T19위친본,구건료포함232개단주적F2군체,고찰웅수일급분지수,이용복합구간작도법진행QTL정위.[결과화결론]결과획득일장포함77개SSR표기적유전련쇄도보,전장868.7 cM,표기평균간거위11.28 cM,공검측도4개여초첨옥미웅수일급분지수상관적QTL위점,분별위우옥미제4、7、8염색체상,가해석5.08%~17.71%적표형변이.주효QTL위점qTBN-4위우제8염색체,가해석17.71%적표형변이.저사QTLs장위웅수일급분지수적분자표기보조선택제공의거.
[Objective]To improve the tassel of maize .[Method]In this study , a cross between sweet corns inbred T4 and T19, which were significantly different in tassel primary branch number , was de-signed and QTLs for tassel primary branch number were identified in a whole genome with the methods of multiple interval mapping (CIM) based on 232 F2 individuals.[Result and conclusion] A genetic link-age map composing of 77 simple sequence repeat ( SSR) markers covering 868.7 cM with an average in-terval of 11.28 cM was constructed.Four QTLs for tassel primary branch number in chromosome 4, 7, 8 were detected , accounting for 5.08%-17.71%of phenotypic variance .The main QTLs were located on chromosome 8 which accounted for 17.71%of the total phenotypic variance .These QTLs provide the ba-sis of marker-assisted selection for tassel primary branch number .