华南农业大学学报
華南農業大學學報
화남농업대학학보
JOURNAL OF SOUTH CHINA AGRICULTURAL UNIVERSITY
2014年
1期
106-109
,共4页
高艳%岳静%黄显会%曾振灵
高豔%嶽靜%黃顯會%曾振靈
고염%악정%황현회%증진령
残留%猪%林可霉素%大观霉素
殘留%豬%林可黴素%大觀黴素
잔류%저%림가매소%대관매소
residue%pig%lincomycin%spectinomycin
[目的]对一种盐酸林可霉素-硫酸大观霉素注射液在猪组织中的残留消除进行研究,并制订该制剂在猪体内的休药期.[方法]常规饲养条件下,将30头健康猪随机分为6组,按猪体质量以15 mg/kg (即林可霉素5 mg/kg和大观霉素10 mg/kg)剂量颈部肌内注射盐酸林可霉素-硫酸大观霉素注射液,每天1次,连用5 d,于最后一次给药后8、24、72、144、240、336 h各宰杀1组猪,分别采集每头猪的肌肉、肝脏、肾脏、脂肪和注射位点5种组织,采用LC-MS/MS法和HPLC-UV法分别对林可霉素和大观霉素进行残留量测定.[结果和结论]林可霉素在猪组织中消除较快,大观霉素消除较慢,给药后第6天所有检测组织中的林可霉素和大观霉素已全部低于最高残留限量,林可霉素和大观霉素在猪体内各组织器官的残留浓度大小顺序均为:肾脏>肝脏>注射部位>肌肉>脂肪,表明肾脏是二者的靶组织.结合农业部规定的林可霉素、大观霉素在猪体内的最高残留限量和WT1.4休药期软件计算结果,建议该制剂在猪体内的休药期为8 d.
[目的]對一種鹽痠林可黴素-硫痠大觀黴素註射液在豬組織中的殘留消除進行研究,併製訂該製劑在豬體內的休藥期.[方法]常規飼養條件下,將30頭健康豬隨機分為6組,按豬體質量以15 mg/kg (即林可黴素5 mg/kg和大觀黴素10 mg/kg)劑量頸部肌內註射鹽痠林可黴素-硫痠大觀黴素註射液,每天1次,連用5 d,于最後一次給藥後8、24、72、144、240、336 h各宰殺1組豬,分彆採集每頭豬的肌肉、肝髒、腎髒、脂肪和註射位點5種組織,採用LC-MS/MS法和HPLC-UV法分彆對林可黴素和大觀黴素進行殘留量測定.[結果和結論]林可黴素在豬組織中消除較快,大觀黴素消除較慢,給藥後第6天所有檢測組織中的林可黴素和大觀黴素已全部低于最高殘留限量,林可黴素和大觀黴素在豬體內各組織器官的殘留濃度大小順序均為:腎髒>肝髒>註射部位>肌肉>脂肪,錶明腎髒是二者的靶組織.結閤農業部規定的林可黴素、大觀黴素在豬體內的最高殘留限量和WT1.4休藥期軟件計算結果,建議該製劑在豬體內的休藥期為8 d.
[목적]대일충염산림가매소-류산대관매소주사액재저조직중적잔류소제진행연구,병제정해제제재저체내적휴약기.[방법]상규사양조건하,장30두건강저수궤분위6조,안저체질량이15 mg/kg (즉림가매소5 mg/kg화대관매소10 mg/kg)제량경부기내주사염산림가매소-류산대관매소주사액,매천1차,련용5 d,우최후일차급약후8、24、72、144、240、336 h각재살1조저,분별채집매두저적기육、간장、신장、지방화주사위점5충조직,채용LC-MS/MS법화HPLC-UV법분별대림가매소화대관매소진행잔류량측정.[결과화결론]림가매소재저조직중소제교쾌,대관매소소제교만,급약후제6천소유검측조직중적림가매소화대관매소이전부저우최고잔류한량,림가매소화대관매소재저체내각조직기관적잔류농도대소순서균위:신장>간장>주사부위>기육>지방,표명신장시이자적파조직.결합농업부규정적림가매소、대관매소재저체내적최고잔류한량화WT1.4휴약기연건계산결과,건의해제제재저체내적휴약기위8 d.
[Objective]The residue depletion of lincomycin hydrochloride-spectinomycin sulfate injection in pigs was investigated in this study , and its withdrawal time was estimated .[Method] In routine breed-ing conditions , thirty healthy pigs were randomly allocated to six treatment groups .All of them were trea-ted with lincomycin hydrochloride-spectinomycin sulfate injection at a dosage of 15 mg/kg ( 5 mg linco-mycin and 10 mg spectinomycin ) once daily for 5 consecutive days .Five medicated pigs were slaughtered at 8, 24, 72, 144, 240 and 336 h after intramuscular administration, and muscle, liver, kidney, fat and injection site tissues were sampled .The tissue concentration of lincomycin and spectinomycin was ana-lyzed respectively by liquid chromatography-mass spsectrometry ( LC-MS/MS) and high-performance liq-uid chromatography ( HPLC ) .[Result and conclusion] At 6 days postmedication , all observations of spectinomycin and lincomycin were below the corresponding MRLs .In all of the detected tissues , kidney had the highest drug concentration followed by liver , injection site , muscle and fat with the lowest drug concentration , indicating that kidney was target tissue for spectinomycin and lincomycin .Based on the MRLs, which were established by the Chinese Agriculture Ministry , the withdrawal time in pigs was ana-lyzed by WT1.4 software.The results indicated that the withdrawal time of lincomycin hydrochloride-spectinomycin sulfate injection should be 8 days after intramuscular administration of 15 mg/kg in pigs.