CAJ | 학술논문

[目的]普通野生稻Oryza rufipogon Griff．蕴含丰富的遗传多样性，对其进行耐冷性数量性状位点（ QTLs）的挖掘和效应分析，可为水稻耐冷性分子育种提供宝贵的基因资源和理论支持．[方法]以籼稻品种9311为受体亲本、普通野生稻品系DP15和DP30为供体亲本，构建染色体片段代换系，鉴定了18个水稻苗期耐冷QTLs，将其中分别包含4个耐冷QTL且遗传背景一致的4个代换系qSCT-1-CSSL、qSCT-4-CSSL、qSCT-8-CSSL和qSCT-12-CSSL分别两两杂交得到2个聚合系（qSCT-1/qSCT-12）-CSSL和（qSCT-4/qSCT-8）-CSSL，对聚合系中各耐冷QTL的互作效应及聚合效应进行研究．[结果和结论]4个耐冷QTL对水稻耐冷性有加性效应；互作分析显示各耐冷QTL 间在聚合系中均存在正向互作．聚合效应在qSCT-4与qSCT-8间表现为QTL间明显的累加效应，而qSCT-1与qSCT-12间聚合的累加效应不明显，表现为qSCT-12对qSCT-1有上位作用．
[목적]보통야생도Oryza rufipogon Griff．온함봉부적유전다양성，대기진행내랭성수량성상위점（ QTLs）적알굴화효응분석，가위수도내랭성분자육충제공보귀적기인자원화이론지지．[방법]이선도품충9311위수체친본、보통야생도품계DP15화DP30위공체친본，구건염색체편단대환계，감정료18개수도묘기내랭QTLs，장기중분별포함4개내랭QTL차유전배경일치적4개대환계qSCT-1-CSSL、qSCT-4-CSSL、qSCT-8-CSSL화qSCT-12-CSSL분별량량잡교득도2개취합계（qSCT-1/qSCT-12）-CSSL화（qSCT-4/qSCT-8）-CSSL，대취합계중각내랭QTL적호작효응급취합효응진행연구．[결과화결론]4개내랭QTL대수도내랭성유가성효응；호작분석현시각내랭QTL 간재취합계중균존재정향호작．취합효응재qSCT-4여qSCT-8간표현위QTL간명현적루가효응，이qSCT-1여qSCT-12간취합적루가효응불명현，표현위qSCT-12대qSCT-1유상위작용．
[Objective]There is a rich genetic diversity in common wild rice , Oryza rufipogon Griff..Ex-ploitation and effective analysis of cold tolerance quantitative trait loci ( QTLs) from the wild rice could provide gene resources and theoretical support for rice cold tolerant molecular breeding .[Method]In our previous study , a total of 18 cold tolerant QTLs distributing throughout all 12 chromosomes of rice genome were discovered using 230 chromosome segment substitution lines ( CSSLs) which were developed from two crosses between the cultivar , 9311 as recipient parent and two lines of core resources of the common wild rice, DP15 and DP30 as donor parents.In this study, the interacting and pyramiding effects of four cold tolerant QTLs qSCT-1, qSCT-4, qSCT-8 and qSCT-12 which were previously identified in the CSSLs were studied using two pyramiding lines , ( qSCT-1/qSCT-12 )-CSSL and ( qSCT-4/qSCT-8 )-CSSL obtained from two crosses between qSCT-1-CSSL and qSCT-12-CSSL, and qSCT-4-CSSL and qSCT-8-CSSL re-spectively against a uniform genetic background .[Result and conclusion]The results indicated that four cold-tolerance QTLs with an additive effect could significantly improve the resistance to rice cold stress separately;there might exist positive interaction effect among these cold tolerance QTLs in pyramiding lines.There was a significant addition effect between qSCT-4 and qSCT-8, but no significant epistasis effect between qSCT-12 and qSCT-1 .