CAJ | 학술논문

[目的]探讨洛克沙胂在鸡饲后的排泄规律及对环境生态的毒性．[方法]选用健康黄鸡饲喂含洛克沙胂50 mg/kg的全价饲料，连续给药10 d，给药期间及停药后收集不同时间点排泄物样，采用微波消解-原子荧光光度法测定鸡排泄物中总砷和无机As（Ⅴ）、As（Ⅲ）质量分数，高效液相色谱法（ HPLC）测定鸡排泄物中洛克沙胂的质量分数．将不同时间点收集的鸡排泄物进行洛克沙胂的光降解试验．[结果和结论]鸡排泄物中砷的排泄量在饲后第108小时质量分数达到稳态（其中洛克沙胂为31.62～35.87 mg/kg，总砷为16.75～21.48 mg/kg），停饲后总砷与洛克沙胂的排泄量呈较快速下降，第120小时时鸡排泄物中已检测不出洛克沙胂，总砷亦降至3.89 mg/kg，此过程中As（Ⅲ）、As（ V）含量变化较小，两者最高值分别为0.38和1.81 mg/kg．光降解试验中光照组鸡排泄物中洛克沙胂的降解率为100％，而避光组降解率仅为75.16％，其中无机As（V）、As（Ⅲ）的质量分数均随时间而升高，并以As （ V）为主，两者在光照下含量变化速度均比相应避光组快．鸡饲喂洛克沙胂后主要以原形形式排泄，无论在避光或光照条件下鸡排泄物中洛克沙胂均可被降解生成无机砷形态，光照可加速鸡排泄物中洛克沙胂的降解．
[목적]탐토락극사신재계사후적배설규률급대배경생태적독성．[방법]선용건강황계사위함락극사신50 mg/kg적전개사료，련속급약10 d，급약기간급정약후수집불동시간점배설물양，채용미파소해-원자형광광도법측정계배설물중총신화무궤As（Ⅴ）、As（Ⅲ）질량분수，고효액상색보법（ HPLC）측정계배설물중락극사신적질량분수．장불동시간점수집적계배설물진행락극사신적광강해시험．[결과화결론]계배설물중신적배설량재사후제108소시질량분수체도은태（기중락극사신위31.62～35.87 mg/kg，총신위16.75～21.48 mg/kg），정사후총신여락극사신적배설량정교쾌속하강，제120소시시계배설물중이검측불출락극사신，총신역강지3.89 mg/kg，차과정중As（Ⅲ）、As（ V）함량변화교소，량자최고치분별위0.38화1.81 mg/kg．광강해시험중광조조계배설물중락극사신적강해솔위100％，이피광조강해솔부위75.16％，기중무궤As（V）、As（Ⅲ）적질량분수균수시간이승고，병이As （ V）위주，량자재광조하함량변화속도균비상응피광조쾌．계사위락극사신후주요이원형형식배설，무론재피광혹광조조건하계배설물중락극사신균가피강해생성무궤신형태，광조가가속계배설물중락극사신적강해．
[Objective]This experiment was conducted to study the excretion of roxarsone in poultry after being fed with roxarsone and its toxicity to the environment .[Method] Thirty yellow plumage meat chick-ens were fed with poultry feed containing roxarsone 50 mg/kg.The feeding trial lasted for 10 days.The excreta were collected at 12 to 240 h after administration and a 5 d withdrawl period .The total arsenic , As(Ⅲ) , As ( V ) in excreta were analyzed by microwave digestion-atomic fluorescence spectrometry ( AFS) method and the roxarsone was analyzed by HPLC .Photo degradation of roxarsone in poultry ex-creta was evaluated .[Result and conclusion]The excreted amount of roxarsone reached a steady-state concentration at 108 h after administration ( the range of roxarsone concentration was 31.62 -35.87 mg/kg; the range of total arsenic concentration was 16.75-21.48 mg/kg) .Roxarsone was undetectable in poultry excreta at 120 h after withdrawal, and the total arsenic was down to 3.89 mg/kg.The quantity of As(Ⅲ) and As (Ⅴ) slightly fluctuated and the peaks were 0.38 and 1.81 mg/kg correspondingly . There was a significant effect of light on the degradation of roxarsone .The results indicated that most of roxarsone was not absorbable and can quickly be eliminated with no change .The degradation rate of rox-arsone can increase the effect of photolysis .