CAJ | 학술논문

[目的]以先前研究所获得的8个与大口黑鲈生长性状相关的分子标记为检测目标，分析其优势基因型在大口黑鲈选育群体中的分布数量，探索生长相关分子标记应用于大口黑鲈基因聚合育种的潜在可行性．[方法]采用STR基因分型技术对大口黑鲈‘优鲈1号’ F2、F3、F5和F6选育群体的生长标记基因型进行检测，8个生长标记中含有4个单核苷酸多态性位点（分别位于IGF-I、POU1F1、PSSⅢ和MSTN基因上）和4个微卫星位点（分别是JZL60、JZL67、MisaTpw76和MisaTpw117）．[结果和结论]试验鱼所含优势基因型的数量为0～6个，F2、F3、F5和F6选育群体中优势基因型的分子标记平均数量依次为2.12、2.70、2.90和3.08，呈现逐代递增趋势；优势基因型的分子标记数量与大口黑鲈生长速度呈同步递增趋势，说明人工选育在一定程度上聚合了优势基因．
[목적]이선전연구소획득적8개여대구흑로생장성상상관적분자표기위검측목표，분석기우세기인형재대구흑로선육군체중적분포수량，탐색생장상관분자표기응용우대구흑로기인취합육충적잠재가행성．[방법]채용STR기인분형기술대대구흑로‘우로1호’ F2、F3、F5화F6선육군체적생장표기기인형진행검측，8개생장표기중함유4개단핵감산다태성위점（분별위우IGF-I、POU1F1、PSSⅢ화MSTN기인상）화4개미위성위점（분별시JZL60、JZL67、MisaTpw76화MisaTpw117）．[결과화결론]시험어소함우세기인형적수량위0～6개，F2、F3、F5화F6선육군체중우세기인형적분자표기평균수량의차위2.12、2.70、2.90화3.08，정현축대체증추세；우세기인형적분자표기수량여대구흑로생장속도정동보체증추세，설명인공선육재일정정도상취합료우세기인．
[Objective]Eight molecular markers related to growth traits were detected in the different breeding populations of largemouth bass , which had been found by previous studies .This would provide useful information for the actual application of limited growth markers in gene pyramiding breeding of largemouth bass .[Method ] Using the technology of STR genotyping , genotype of 8 growth markers in the F2, F3, F5 and F6 generations of largemouth bass (Micropterus salmoides),‘Youlu No.1’ were de-tected and the number of advantage genotypes was analyzed .The growth markers included four sites of single nucleotide polymorphism located in IGF-I, POU1F1, PSSⅢand MSTN gene and four sites of mic-rosatellite:JZL60, JZL67, MisaTpw76 and MisaTpw117.[Result and conclusion] The number of ad-vantage genotypes was 0-6 in the tested fishes.The average number was 2.12, 2.70, 2.90 and 3.08 in F2, F3, F5 and F6 generations, respectively, presenting an increasing trend with each generation .The number of the advantage genotypes increased synchronously with the growth rate of the breeding large -mouth bass in different populations .This means that the artificial selection has polymerized the advantage genotype to some extent .