CAJ | 학술논문

目的：建立 DBA /1小鼠胶原性关节炎模型（CIA ），分析其发病的特点、临床表现及病理学变化。方法用完全弗氏佐剂乳化牛Ⅱ型胶原，在 DBA/l 小鼠尾根部皮内注射100μL ，3周后重复注射等量抗原，动态观察小鼠的临床变化，如足爪肿胀程度、关节炎指数评分和体重变化等，对发病关节及小鼠脾脏进行病理学检测。结果从首次免疫后28 d 开始，胶原免疫组小鼠踝关节开始出现明显红肿，7周左右肿胀达到高峰，小鼠 CIA 发生率为100％；病理学结果显示，患病关节出现炎性细胞浸润、软骨和骨骼破坏、滑膜增生等较为典型的变化；脾脏生发中心增多，红髓充血；正常对照组小鼠则无上述表现。结论用牛Ⅱ型胶原，可成功诱导 DBA /l 小鼠 CIA 模型，发生率为100％。该模型较充分模拟了人类 RA 的病变特征，关节炎指数、踝关节和脾脏病理等是评价 CIA 模型的主要指标。
목적：건립 DBA /1소서효원성관절염모형（CIA ），분석기발병적특점、림상표현급병이학변화。방법용완전불씨좌제유화우Ⅱ형효원，재 DBA/l 소서미근부피내주사100μL ，3주후중복주사등량항원，동태관찰소서적림상변화，여족조종창정도、관절염지수평분화체중변화등，대발병관절급소서비장진행병이학검측。결과종수차면역후28 d 개시，효원면역조소서과관절개시출현명현홍종，7주좌우종창체도고봉，소서 CIA 발생솔위100％；병이학결과현시，환병관절출현염성세포침윤、연골화골격파배、활막증생등교위전형적변화；비장생발중심증다，홍수충혈；정상대조조소서칙무상술표현。결론용우Ⅱ형효원，가성공유도 DBA /l 소서 CIA 모형，발생솔위100％。해모형교충분모의료인류 RA 적병변특정，관절염지수、과관절화비장병리등시평개 CIA 모형적주요지표。
Objective To estahlish a collagen-induced arthritis ( CIA ) model with DBA / 1 mice and detect its characteristics .Methods The CIA mouse model was established by immunization of DBA/ 1 mice with cattle collagen Ⅱ . DBA /1 mice were injected intradermally at the base of the tail with 100μLemulsion containing an equal volume of cattle type Ⅱ collagen solution and CFA .The second injection was repeated 3 weeks after the first injection . The features of CIA in joints swelling , inflammatory index , body weight and pathological changes were investigated .Results Joints swelling of mice began to appear at 28 days ,and were most severe after 7 weeks .There were infiltrating inflammatory cells surrounding the vascellum ,synovium hyperplasy , joints destruction and spleen changing indicated by pathological examination . The incidence of CIA in DBA/ 1 mice could reach 100% . Conclusion An animal model of CIA can be induced in DBA/ 1 mice at a very high incidence .The CIA model could mimic the characteristics of human rheumatoid arthritis . The inflammatory index , the pathology of ankle joints and spleen were major index to evaluate CIA .