包装学报
包裝學報
포장학보
PACKAGING JOURNAL
2013年
4期
34-38
,共5页
罗洁%郝喜海%李贞%衣潇鹏
囉潔%郝喜海%李貞%衣瀟鵬
라길%학희해%리정%의소붕
类产碱假单胞菌%PVA%降解率%吸光度
類產堿假單胞菌%PVA%降解率%吸光度
류산감가단포균%PVA%강해솔%흡광도
pseudomonas pseudoalcaligenes%PVA%degradation rate%absorbance
对类产碱假单胞菌降解不同型号PVA(0588,1788,2088,1750,1799)的降解效果及降解特性进行了研究。采用UV2900紫外分光光度计测吸光度法测定了各型号PVA的降解率,同时研究了PVA的分子量和醇解度、PVA浓度、接种菌种浓度、摇瓶转速等对PVA降解率的影响。结果表明:醇解度一定时,高聚合度PVA的降解效率远低于低聚合度PVA;而聚合度一定时,醇解度对降解率的影响不明显。在培养温度为30℃,培养时间为72 h,摇瓶转速为180 rad/min,PVA的质量浓度为0.5 g/L,接种菌的体积分数为4.0%时, PVA的降解率可达90.15%。
對類產堿假單胞菌降解不同型號PVA(0588,1788,2088,1750,1799)的降解效果及降解特性進行瞭研究。採用UV2900紫外分光光度計測吸光度法測定瞭各型號PVA的降解率,同時研究瞭PVA的分子量和醇解度、PVA濃度、接種菌種濃度、搖瓶轉速等對PVA降解率的影響。結果錶明:醇解度一定時,高聚閤度PVA的降解效率遠低于低聚閤度PVA;而聚閤度一定時,醇解度對降解率的影響不明顯。在培養溫度為30℃,培養時間為72 h,搖瓶轉速為180 rad/min,PVA的質量濃度為0.5 g/L,接種菌的體積分數為4.0%時, PVA的降解率可達90.15%。
대류산감가단포균강해불동형호PVA(0588,1788,2088,1750,1799)적강해효과급강해특성진행료연구。채용UV2900자외분광광도계측흡광도법측정료각형호PVA적강해솔,동시연구료PVA적분자량화순해도、PVA농도、접충균충농도、요병전속등대PVA강해솔적영향。결과표명:순해도일정시,고취합도PVA적강해효솔원저우저취합도PVA;이취합도일정시,순해도대강해솔적영향불명현。재배양온도위30℃,배양시간위72 h,요병전속위180 rad/min,PVA적질량농도위0.5 g/L,접충균적체적분수위4.0%시, PVA적강해솔가체90.15%。
The degradation effects and characters of Pseudomonas Pseudoalcaligenes with PVA(0588, 1788, 2088, 1750, 1799) were studied. The degradation rates of different types of PVA were measured with UV2900 ultraviolet spectrophotometer. The molecular weight and degree of alcoholysis,PVA concentration, concentration of inoculated strains, rotary speed effects on PVA degradation rate were researched as well. The optimal conditions were determined as:when the culture temperature was 30℃,with the treating time of 72 h, rotary speed of 180rad/min, the concentration of PVA as 0.5 g/L and the concentration of pseudomonas pseudoalcaligenes as 4.0%, the ratio of degradation could reach as high as 90.15%.