CAJ | 학술논문

目的：通过对发热伴血小板减少综合征布尼亚病毒（简称“新布尼亚病毒”）进行规模化生产培养工艺研究，为新布尼亚病毒规模化生产提供有力支持。方法采用细胞工厂培养Vero细胞，待其长成致密单层后，取工作种子批新布尼亚病毒毒种接种细胞，采用连续收获或细胞病变充分时收获培养液上清的方法收获病毒，并以病毒滴度、抗原含量作为评价指标选择基础培养基、培养基pH、人血白蛋白添加浓度、接种细胞日龄、接种病毒MOI以及病毒培养温度。结果按0．01～0．001 MOI接种3～4日龄Vero细胞，病毒培养液选择含0．3％人血白蛋白pH 7．6～7．8的DMEM溶液，35℃培养7 d收获，病毒收获液病毒滴度7．87 LgCCID50/mL、抗原含量170．1μg/mL。结论初步建立了新布尼亚病毒规模化生产培养工艺，为后续工业化生产提供了数据支持。
목적：통과대발열반혈소판감소종합정포니아병독（간칭“신포니아병독”）진행규모화생산배양공예연구，위신포니아병독규모화생산제공유력지지。방법채용세포공엄배양Vero세포，대기장성치밀단층후，취공작충자비신포니아병독독충접충세포，채용련속수획혹세포병변충분시수획배양액상청적방법수획병독，병이병독적도、항원함량작위평개지표선택기출배양기、배양기pH、인혈백단백첨가농도、접충세포일령、접충병독MOI이급병독배양온도。결과안0．01～0．001 MOI접충3～4일령Vero세포，병독배양액선택함0．3％인혈백단백pH 7．6～7．8적DMEM용액，35℃배양7 d수획，병독수획액병독적도7．87 LgCCID50/mL、항원함량170．1μg/mL。결론초보건립료신포니아병독규모화생산배양공예，위후속공업화생산제공료수거지지。
Objective To investigate the ideal culture process of SFTS Bunyavirus and provide the basic date for scale pro-duction.Methods Vero cells were cultured using cell factory .The working seed of SFTS bunyavirus was inoculated into Vero cells when the cells grew into monolayer .The virus were harvested with continuous perfused culture system or harves-ting the supernatant of culture when the cells achieved fully lesions .The virus titers and antigen contents were used as indi-cators for selecting medium , pH of medium , added human serum albumin concentration ,virus incubation temperature ,cells age and MOI of virus.Results 3-4 days old Vero cells were inoculated with 0.01-0.001,MOI of SFTS Bunyavirus, DMEM solution containing 0.3%human serum albumin was selected as the medium (pH 7.6-7.8), the virus were cul-tured at 35 ℃for 7 days, the harvested virus titer is 7.87 LgCCID50/mL and the antigen content is 170.1 μg/mL.Con-clusion The scale culture process of SFTS Bunyavirus was initially established , and it laid a good foundation for industrial production .