CAJ | 학술논문

选取在山东省济宁地区大豆产区曾经或正在生产上推广使用的35个品种为供试品种，采用SDS-PAGE电泳技术测定提取蛋白质11S组分、7S组分、亚基组和亚基的相对含量以及亚基数量，研究其变异特点.在济宁地区同一条件下的结果表明：（1）11S和7S组分的亚基总数变异范围为15~23个，平均为18.03个.7S组分亚基数量变异范围为3~7个，平均4.49个，有5个亚基组存在不同程度的缺失.11S组分亚基数量变异范围为11~19个，平均13.54个，没有亚基组缺失现象.（2）7S组分相对含量的变异范围为14.78~27.54%，平均为22.07%；11S组分的变异范围为49.04~63.52%，平均为59.77%.11S/7S比值变异范围为2.12~4.11，平均为2.77.（3）7S组分6个亚基组的相对含量平均为4.38~6.62%，11S组分4个亚基组平均相对含量为7.02~21.72%.（4）7S组分相对含量与11S组分之间有微弱的正相关（r=0.08），7S组分相对含量与11S/7S比值之间存在极显著的负相关（r=-0.94）.而11S组分相对含量与11S/7S比值之间存在微弱的正相关（r=0.19）.7S的6个亚基组中相邻的2个亚基组之间都存在显著或极显著的负相关（r=-0.38~-0.85）.11S组分的相邻亚基组之间存在负相关关系（r=-0.78~-0.95），不相邻的亚基组之间相关关系微弱.
선취재산동성제저지구대두산구증경혹정재생산상추엄사용적35개품충위공시품충，채용SDS-PAGE전영기술측정제취단백질11S조분、7S조분、아기조화아기적상대함량이급아기수량，연구기변이특점.재제저지구동일조건하적결과표명：（1）11S화7S조분적아기총수변이범위위15~23개，평균위18.03개.7S조분아기수량변이범위위3~7개，평균4.49개，유5개아기조존재불동정도적결실.11S조분아기수량변이범위위11~19개，평균13.54개，몰유아기조결실현상.（2）7S조분상대함량적변이범위위14.78~27.54%，평균위22.07%；11S조분적변이범위위49.04~63.52%，평균위59.77%.11S/7S비치변이범위위2.12~4.11，평균위2.77.（3）7S조분6개아기조적상대함량평균위4.38~6.62%，11S조분4개아기조평균상대함량위7.02~21.72%.（4）7S조분상대함량여11S조분지간유미약적정상관（r=0.08），7S조분상대함량여11S/7S비치지간존재겁현저적부상관（r=-0.94）.이11S조분상대함량여11S/7S비치지간존재미약적정상관（r=0.19）.7S적6개아기조중상린적2개아기조지간도존재현저혹겁현저적부상관（r=-0.38~-0.85）.11S조분적상린아기조지간존재부상관관계（r=-0.78~-0.95），불상린적아기조지간상관관계미약.
The relative content of 11S and 7S composition, their subunit groups and subunits in protein extracted of 35 summer soybean varieties to be used in Jining area, Shandong province were tested by using SDS-PAGE analysis. The results obtained under environment conditions in Janjing are as follows:(1) The subunit total amount of 7S were from 3 to 7, mean was 4.49. There existed various lack among 5 subunit groups in 7S. The subunit total amount of 11S were from 11 to 19, mean was 13.54. There didn’t existed lack of subunit groups in 11S. (2) The mean relative content of 7S was 22.7%, with a range of 14.8%~27.54%.The mean relative content of 11S was 59.77%, with a range of 49.04%~63.52%. That of 11S/7S ratio was 2.77 with a range of 2.12~4.11. (3) The mean relative content of 6 subunit groups in 7S were from 4.38%to 6.62%.The mean relative content of 4 subunit groups in 11S were from7.02%to 21.72%.(4) The correlation between relative content of 7S and 11S was low (r=0.08), between relative content of 7S and 11S/7S ratio was high (r=-0.94). The correlation coefficient of border subunit groups were from-0.38 to-0.85 in 7S, and from-0.78 to-0.95 in 11S, respectively.