CAJ | 학술논문

建立了两性离子亲水作用色谱/质谱联用方法用于大肠杆菌胞内极性代谢物的分离分析。选取52个代表性极性物质对方法进行考察，发现此方法有较好的线性范围，且大部分物质最低检测限均在 ng/mL 数量级。平行制备6份样品进行分析，结果显示85％以上代谢物峰面积的 RSD值小于30％。6个内标物质在低、中、高3个浓度下的日内精密度（ RSD）均小于20％，大部分物质的相对回收率都在可接受的范围内（70％~130％）。把此方法用于yfcC基因改造的3株大肠杆菌代谢组分析，发现一些小肽、氨基酸、核苷、有机酸、磷脂等物质在基因改造后发生明显变化。此研究结果表明，建立的两性离子亲水作用色谱/质谱方法检测到的物质化学性质分布广，跨越了极性磷脂到小肽的各个范围，且具有良好的重复性、稳定性和适用性。
건립료량성리자친수작용색보/질보련용방법용우대장간균포내겁성대사물적분리분석。선취52개대표성겁성물질대방법진행고찰，발현차방법유교호적선성범위，차대부분물질최저검측한균재 ng/mL 수량급。평행제비6빈양품진행분석，결과현시85％이상대사물봉면적적 RSD치소우30％。6개내표물질재저、중、고3개농도하적일내정밀도（ RSD）균소우20％，대부분물질적상대회수솔도재가접수적범위내（70％~130％）。파차방법용우yfcC기인개조적3주대장간균대사조분석，발현일사소태、안기산、핵감、유궤산、린지등물질재기인개조후발생명현변화。차연구결과표명，건립적량성리자친수작용색보/질보방법검측도적물질화학성질분포엄，과월료겁성린지도소태적각개범위，차구유량호적중복성、은정성화괄용성。
The zwitterionic hydrophilic interaction liquid chromatography coupled with mass spectrometry( ZIC-HILIC/MS)method was developed and applied for studying the metabolome of Escherichia coli. In this method,the linear ranges of the 52 representative compounds were between 2-6 orders of magnitude. Moreover the lowest limits of detection of most compounds were at ng/mL levels. The analysis for six samples prepared in parallel showed that the method had good reproducibility with more than 85% metabolites with the RSDs of peak area less than 30%. The precision of the method was tested by evaluating the relative recoveries of six internal standards at the low,middle and high concentrations. The results exhibited that the intraday precisions( RSDs)of the six compounds in the same concentrations were less than 20%. The recoveries of most compounds were accepted in the range of 70%-130%. The results of metabo-lomic analysis of three Escherichia coli strains with genetic modified yfcC gene showed that some small peptides, amino acids, nucleosides, organic acids and phospholipids changed differentially after the yfcC gene modification. These results indicated that the ZIC-HILIC/MS method could detect diverse metabolites and be used for metabolomic analysis purpose with good reproducibility and stability.