CAJ | 학술논문

应用可逆加成-断裂链转移（ RAFT）策略制备了一种抗原决定基表面印迹微球。这一工作以转铁蛋白的抗原决定基 N端九肽作为模板，通过共价键合的方式固载于修饰了戊二醛的硅胶颗粒表面。然后以甲基丙烯酸、甲基丙烯酰羟乙酯为功能单体，甲叉基双丙烯酰胺为交联剂，偶氮二异丁腈（ AIBN）为引发剂，N，N-二甲基甲酰胺为溶剂，在三硫酯试剂2-（十二烷基三硫代碳酸酯基）-2-甲基丙酸的调控下，于70℃进行活性-可控的聚合反应，制备得到分子印迹微球。该材料对模板抗原决定基的识别容量为2.36 mg/g，印迹因子为1.89；对转铁蛋白的识别容量为4.98 mg/g，印迹因子为1.61，120 min内可达到吸附平衡；在多蛋白质竞争识别中，该材料对转铁蛋白识别的印迹因子远高于细胞色素C、乳球蛋白等其他竞争蛋白质的印迹因子。以上结果证明，通过RAFT策略制备得到的抗原决定基分子印迹材料在对抗原决定基具有良好的识别能力的同时，对模板抗原决定基对应的转铁蛋白也具有优良的选择性、较高的识别容量和较快的识别速度。
응용가역가성-단렬련전이（ RAFT）책략제비료일충항원결정기표면인적미구。저일공작이전철단백적항원결정기 N단구태작위모판，통과공개건합적방식고재우수식료무이철적규효과립표면。연후이갑기병희산、갑기병희선간을지위공능단체，갑차기쌍병희선알위교련제，우담이이정정（ AIBN）위인발제，N，N-이갑기갑선알위용제，재삼류지시제2-（십이완기삼류대탄산지기）-2-갑기병산적조공하，우70℃진행활성-가공적취합반응，제비득도분자인적미구。해재료대모판항원결정기적식별용량위2.36 mg/g，인적인자위1.89；대전철단백적식별용량위4.98 mg/g，인적인자위1.61，120 min내가체도흡부평형；재다단백질경쟁식별중，해재료대전철단백식별적인적인자원고우세포색소C、유구단백등기타경쟁단백질적인적인자。이상결과증명，통과RAFT책략제비득도적항원결정기분자인적재료재대항원결정기구유량호적식별능력적동시，대모판항원결정기대응적전철단백야구유우량적선택성、교고적식별용량화교쾌적식별속도。
A kind of novel epitope surface imprinted particles was prepared by the reversible addition fragmentation chain transfer( RAFT)strategy. The epitope of transferrin,N-terminal peptide of the protein with nine amino acid residues,was chosen as the template and immobi-lized with covalent interaction on the surface of silica particles through the truss arm glutaralde-hyde. The living/controlled polymerization was initialed by 2,2′-azobisisobutyronitrile( AIBN) at 70 ℃ in the solution of N,N-dimethylformamide,with the regulation by triothioester agent 2-( dodecylthiocarbonothioylthio )-2-methylpropanoic acid. Methacrylic acid and 2-hydroxyethyl methacrylate were chosen as the functional monomers and N,N-methylenebisacrylamide was chosen as the cross-linker in this polymerization. For this material,the binding capacity of the nine residue peptide could reach 2. 36 mg/g with the imprinting factor( IF)of 1. 89,while that for transferrin could reach 4. 98 mg/g with IF of 1. 61. The equilibrium could be achieved in 120 min for the transferrin recognition. In multi-protein competitive recognition,the imprinted fac-tor of transferrin was the highest in the mixture of transferrin and other competitive proteins , such as cytochrome C and β-lactoglobulin. The results indicated that these epitope surface imprinted particles with RAFT strategy could recognize not only the nine residue peptide but also the transferrin with good selectivity,high binding capacity and fast mass transfer.