CAJ | 학술논문

在流感病毒研究的动物模型中，雪貂（Ferret）是目前最为敏感的实验动物，鼻内接种人流感病毒后出现与人相似的感染症状，广泛用于流感病毒的机制、传播和疫苗评价等研究。对雪貂进行正确和规范的操作是影响试验结果成功与否的关键因素之一，当前国内尚无在流感病毒研究中对雪貂操作的实验技术进行系统详细的报道。论文结合实际操作和文献对流感病毒研究中雪貂的抓取、麻醉、病毒接种、鼻洗液收集、心脏采血、临床症状观察、剖检和组织样品保存等操作流程进行探索性研究，初步建立了标准规范的实验操作程序，具有较强的实用性和可操作性，对后续研究者具有重要的参考和指导意义。
재류감병독연구적동물모형중，설초（Ferret）시목전최위민감적실험동물，비내접충인류감병독후출현여인상사적감염증상，엄범용우류감병독적궤제、전파화역묘평개등연구。대설초진행정학화규범적조작시영향시험결과성공여부적관건인소지일，당전국내상무재류감병독연구중대설초조작적실험기술진행계통상세적보도。논문결합실제조작화문헌대류감병독연구중설초적조취、마취、병독접충、비세액수집、심장채혈、림상증상관찰、부검화조직양품보존등조작류정진행탐색성연구，초보건립료표준규범적실험조작정서，구유교강적실용성화가조작성，대후속연구자구유중요적삼고화지도의의。
Ferrets play an important role in influenza virus research,and the ferrets are used as an animal model for the study of influenza because they are most susceptible to human influenza viruses and develop some of the symptoms of influenza that are similar with humans.The ferrets are ideal animal models in the research of mechanism,transmission and vaccine evaluation of influenza virus.The correct and standard operations have important influence to the result of the research.Here,we carried on the exploratory re-search of influenza virus infection model in ferrets about grabbing,anesthesia,virus infection,nasal wash and heart blood collection,clinical symptom record and tissue harvest according to reported actual opera-tion and relevant literature,and built the standard laboratory procedures preliminary.The research has strong practicability and manipuility,and important reference value for other researchers.