CAJ | 학술논문

应用 HE 染色方法观察罗非鱼口服免疫不同剂量无乳链球菌弱毒苗后不同肠段的肠绒毛形态、肠上皮杯状细胞及肠上皮内淋巴细胞的数量及分布。免疫20 d 后对所有剩余的罗非鱼均以1．67×107 CFU/尾无乳链球菌进行攻毒，攻毒后10 d 计算相对保护率。结果显示，免疫组与对照组罗非鱼各肠段肠绒毛长度无明显差异，免疫组肠道杯状细胞数量较对照组均减少，且差异极显著（P ＜0．01）；各免疫组后肠段上皮内淋巴细胞数量明显增加，且与对照组相比差异显著或极显著（P ＜0．05或 P ＜0．01）；攻毒10 d 后，对照组死亡率最高，为90．00％，而各免疫组均获得不同程度的免疫保护，最高可达66．67％。说明口服无乳链球菌弱毒疫苗可以提高罗非鱼对无乳链球菌的抵抗力，且以1．0×109 CFU/mL 的剂量免疫后获得的保护率最高。
응용 HE 염색방법관찰라비어구복면역불동제량무유련구균약독묘후불동장단적장융모형태、장상피배상세포급장상피내림파세포적수량급분포。면역20 d 후대소유잉여적라비어균이1．67×107 CFU/미무유련구균진행공독，공독후10 d 계산상대보호솔。결과현시，면역조여대조조라비어각장단장융모장도무명현차이，면역조장도배상세포수량교대조조균감소，차차이겁현저（P ＜0．01）；각면역조후장단상피내림파세포수량명현증가，차여대조조상비차이현저혹겁현저（P ＜0．05혹 P ＜0．01）；공독10 d 후，대조조사망솔최고，위90．00％，이각면역조균획득불동정도적면역보호，최고가체66．67％。설명구복무유련구균약독역묘가이제고라비어대무유련구균적저항력，차이1．0×109 CFU/mL 적제량면역후획득적보호솔최고。
The common histological HE staining method was used to study the morphology of intestinal vil-li,the distribution and abundance of intestinal goblet cells and intraepithelial lymphocytes of tilapia which were immunized with different concentrations of oral attenuated vaccine of Streptococcus agalactiae .20 days after immunization,the remainder were challenged with 1.67×10 7 CFU/mL of S.agalactiae,and 10 days after challenge,the RPS were observed.15 days after immunization,the immunized groups showed no significantiy differences in the length of intestinal villi compared with the control group(P > 0.05);the quanlity of goblet cell in the immunized groups were significantly decreased than those in control group(P<0.01 );and the quanlity of intraepithelial lymphocytes in the midgut and hindgut of the immunized groups were significantly increased than those in control group (P <0.05 or P <0.01).By 10 days post-challenge,the highest mortality was found in the control group(90.00%),however,all of the immunized groups obtained different degrees of immune protection,and the highest RPS was found in the 1.0×10 9 CFU/mL group(66.67%).Oral attenuated vaccine of S.agalactiae can enhance the resistance of tilapia, and the best immune effect was observed in the group of 1.0×10 9 CFU/mL.