CAJ | 학술논문

为了解健康禽源大肠埃希菌的耐药性及其Ⅰ型和Ⅱ型整合子携带情况和流行特征，探讨整合子与大肠埃希菌耐药表型的相关性，分析整合子在细菌多重耐药中的作用，采用 MIC 法和 PCR 方法分别检测249株健康禽源大肠埃希菌的耐药性和Ⅰ型、Ⅱ型整合酶基因及可变区。结果显示，12种药物耐药率均超过56．63％，多重耐药率高达98．39％；245株多重耐药菌株均含有Ⅰ型整合子，但含有Ⅱ型整合子的大肠埃希菌仅113株，Ⅰ型和Ⅱ型整合子的检出率分别为100％和46．12％；63．75％的Ⅰ型整合子阳性菌株扩增出5类大小不同的基因盒，大小为1000 bp～2300 bp，18．6％的Ⅱ型整合子阳性菌株扩增出2200 bp 的基因盒；58．82％的菌株携带2种或2种以上的基因盒，基因盒以编码甲氧苄啶类和氨基糖苷类药物耐药性的 dfrA和 aadA 基因家族为主，其中 aadA22为优势基因盒，其次是 dfrA17＋aadA5基因盒。说明胶东地区禽源大肠埃希菌多重耐药现象严重；Ⅰ型整合子比Ⅱ型整合子更流行；Ⅰ型和Ⅱ型整合子参与多重耐药，但细菌携带的整合子-基因盒与其耐药表型无对应关系。
위료해건강금원대장애희균적내약성급기Ⅰ형화Ⅱ형정합자휴대정황화류행특정，탐토정합자여대장애희균내약표형적상관성，분석정합자재세균다중내약중적작용，채용 MIC 법화 PCR 방법분별검측249주건강금원대장애희균적내약성화Ⅰ형、Ⅱ형정합매기인급가변구。결과현시，12충약물내약솔균초과56．63％，다중내약솔고체98．39％；245주다중내약균주균함유Ⅰ형정합자，단함유Ⅱ형정합자적대장애희균부113주，Ⅰ형화Ⅱ형정합자적검출솔분별위100％화46．12％；63．75％적Ⅰ형정합자양성균주확증출5류대소불동적기인합，대소위1000 bp～2300 bp，18．6％적Ⅱ형정합자양성균주확증출2200 bp 적기인합；58．82％적균주휴대2충혹2충이상적기인합，기인합이편마갑양변정류화안기당감류약물내약성적 dfrA화 aadA 기인가족위주，기중 aadA22위우세기인합，기차시 dfrA17＋aadA5기인합。설명효동지구금원대장애희균다중내약현상엄중；Ⅰ형정합자비Ⅱ형정합자경류행；Ⅰ형화Ⅱ형정합자삼여다중내약，단세균휴대적정합자-기인합여기내약표형무대응관계。
In order to understand the drug resistance of healthy poultry E.coli and its Ⅰ and Ⅱ integron carried and popular features,discuss the correlation of integron and E.coli drug-resistant phenotype,ana-lyze of the role of integron in multiple drug-resistant bacteria,MIC method and polymerase chain reaction (PCR)were used to determine of antimicrobial drug resistance andⅠ,Ⅱ integrase genes and variable area of 249 healthy poultry E.coli respectively,and the enzyme digestion,sequencing analysis of the resistance gene cassettes carried by integron variable area were made.The results showed that the resistant rate of 12 kinds of drugs was more than 56.63%,multiple resistant rate was as high as 98.39%;245 multiple drug-resistant strains all contained Ⅰ integron,but only 113 strains E.coli contained Ⅱ integron,the detection rates ofⅠ and Ⅱ integron were 100% and 46.12%,respectively ;63.75% ofⅠ integron positive strains amplified out 5 kinds of different size gene cassettes,the size was 1 000-2 300 bp,18.6% ofⅡ integron positive strains amplified out 2 200 bp gene cassette;58.82% of strains carried two or more than two gene cassettes,gene cassettes were priority to encode methoxyl benzyl organism and aminoglycoside drug resist-ant dfrA and aadA gene families,among them aad A22 as dominant gene cassette,followed by dfrA17 +aadA5 gene cassettes.The results indicate that the multiple drug-resistance of poultry E.coli from Jia-odong region is serious;Ⅰ integron is more popular than Ⅱ integron,Ⅰ and Ⅱ integron both participate in multiple drug resistance,but integron-gene cassette carried by the bacteria has no corresponding relation with its drug-resistant phenotype.