CAJ | 학술논문

为了解奶牛乳腺炎病例乳汁中白细胞介素-6（IL-6）与体细胞凋亡的相关性，探讨了患有不同程度乳腺炎的奶牛乳汁中 IL-6水平变化，并研究了 IL-6与乳汁体细胞凋亡的相关性。利用无菌消毒法对某奶牛饲养场的82头奶牛采集乳样，采用放射免疫分析法测定乳清 IL-6，应用流式细胞仪检测乳汁体细胞凋亡率。结果表明，隐性乳腺炎乳汁中 IL-6水平极显著高于无乳腺炎乳汁中 IL-6水平（P ＜0．01），临床乳腺炎乳汁中 IL-6水平极显著高于无乳腺炎乳汁和隐性乳腺炎乳汁中 IL-6水平（P ＜0．01）；奶牛乳腺炎病例乳汁中 IL-6水平与上皮细胞、巨噬细胞、淋巴细胞和中性粒细胞凋亡呈明显负相关（P ＜0．01），其中 IL-6与中性粒细胞的相关系数接近1或达到1，二者的相关性最明显，IL-6对中性粒细胞凋亡抑制最明显。奶牛乳汁中 IL-6水平与奶牛乳腺炎的发病程度密切相关，IL-6不同程度的抑制牛乳汁中体细胞的凋亡过程，IL-6对中性粒细胞的凋亡过程的明显抑制与奶牛乳腺炎有重要关系。
위료해내우유선염병례유즙중백세포개소-6（IL-6）여체세포조망적상관성，탐토료환유불동정도유선염적내우유즙중 IL-6수평변화，병연구료 IL-6여유즙체세포조망적상관성。이용무균소독법대모내우사양장적82두내우채집유양，채용방사면역분석법측정유청 IL-6，응용류식세포의검측유즙체세포조망솔。결과표명，은성유선염유즙중 IL-6수평겁현저고우무유선염유즙중 IL-6수평（P ＜0．01），림상유선염유즙중 IL-6수평겁현저고우무유선염유즙화은성유선염유즙중 IL-6수평（P ＜0．01）；내우유선염병례유즙중 IL-6수평여상피세포、거서세포、림파세포화중성립세포조망정명현부상관（P ＜0．01），기중 IL-6여중성립세포적상관계수접근1혹체도1，이자적상관성최명현，IL-6대중성립세포조망억제최명현。내우유즙중 IL-6수평여내우유선염적발병정도밀절상관，IL-6불동정도적억제우유즙중체세포적조망과정，IL-6대중성립세포적조망과정적명현억제여내우유선염유중요관계。
In order to understand the correlation between interleukin-6 (IL-6)and somatic cell apoptosis in mastitis cow milk,we studied the level changes of IL-6 in milks from cows with varying degrees of masti-tis,and then the correlation of IL-6 and somatic cell apoptosis.82 samples were collected aseptically,IL-6 was measured using radioimmunoassay and the apoptosis was detected by flow cytometry.The results showed that the IL-6 levels in milks from recessive mastitis cases were significantly higher than those in normal cases (P <0.01),the IL-6 levels from dominant mastitis cases were significantly higher than that from recessive mastitis cases and normal cases (P <0.01).The IL-6 levels in mastitis cases were signifi-cantly negatively correlated with apoptosis of epithelial cells,macrophages,lymphocytes,and neutrophil (P <0.01).The most obvious correlation were found between IL-6 and neutrophils of which the correlation coefficient were closed 1 or up to 1,and IL-6 suppressed the apoptosis of neutrophils most obviously.The IL-6 levels in cow milk is closely related to the degree of mastitis,IL-6 inhibits apoptosis of somatic cells in different degrees,and the apoptosis process inhibition of IL-6 on neutrophils has an important relationship with mastitis.