CAJ | 학술논문

本文介绍了LAMP?技术核酸快速诊断技术平台建立的流程，解决了LAMP?法易污染、非特异扩增、无法定量、无法高通量等问题，并将RT-LAMP?技术应用于H7N9疫情的检测，灵敏度能达到10copy/μl，比RT-PCR高100倍，整个反应时间更短，比RT-PCR节约45min。LAMP?法具有快速、恒温、简易、高特异、高敏感性的优点，特别适用于现场、基层医疗单位的快速检测。
본문개소료LAMP?기술핵산쾌속진단기술평태건립적류정，해결료LAMP?법역오염、비특이확증、무법정량、무법고통량등문제，병장RT-LAMP?기술응용우H7N9역정적검측，령민도능체도10copy/μl，비RT-PCR고100배，정개반응시간경단，비RT-PCR절약45min。LAMP?법구유쾌속、항온、간역、고특이、고민감성적우점，특별괄용우현장、기층의료단위적쾌속검측。
This paper introduces the platform establishment of LAMP? rapid nucleic acid detection technology, and set le the problem of law easy pol ution, non-speciifc ampliifcation,can?t quantitative, high-throughput can?t be realized. And apply RT-LAMP? technology to H7N9 detection, the sensitivity can reached 10 copy/μl, 100 times higher than RT-PCR, and the reaction time is 45min shorter than the RT-PCR. So the LAMP? method is rapid, isothermal, simple, high speciifcity and high sensitivity, and it is particularly suitable for rapid detection on ifeld and primary health care units.