CAJ | 학술논문

　　定性分析酸枣仁黄酮提取物在大鼠胆汁中代谢成分。大鼠口服酸枣仁黄酮提取物后行胆管插管术，于0-34h 内收集胆汁，采用高效液相色谱-离子阱质谱法定性分析其代谢物成分。结果显示胆汁中两种原形成分和四种代谢陈分得到推定。 HPLC -DAD -MSn 可用于定性分析酸枣仁黄酮提取物在大鼠体内的代谢过程，其代谢成分主要以原形形式存在，并可能与其代谢产物协同内源性成分发挥药理活性。
　　정성분석산조인황동제취물재대서담즙중대사성분。대서구복산조인황동제취물후행담관삽관술，우0-34h 내수집담즙，채용고효액상색보-리자정질보법정성분석기대사물성분。결과현시담즙중량충원형성분화사충대사진분득도추정。 HPLC -DAD -MSn 가용우정성분석산조인황동제취물재대서체내적대사과정，기대사성분주요이원형형식존재，병가능여기대사산물협동내원성성분발휘약리활성。
The aim of this study was to establish a method for qualitative analysis of the metabolites in rat bile after oral administration of Semen Ziziphi Spinosae flavonoid extract .A cystic duct encheiresis was carried out on a SD rat to collect the bile from zero to 34th hour after oral adminis-tration of Semen Ziziphi Spinosae flavonoid extract .High performance liquid chromatography -ion -trap mass spectrometry was applied for qualita-tive analysis of metabolite composition .The results showed that two original flavonoids (spinosin and 6?-feruloylspinosin) and four metabolic com-ponents in the bile samples were detected .The method of HPLC - Diode - Array Detection (DAD) and Ion - Trap Mass Spectrometry (MSn) (HPLC -DAD -MSn) could be used to qualitatively analyze the metabolism of Semen Ziziphi Spinosae flavonoid extract in rat .Original flavonoids are the main metabolite compositions ,and the pharmacological activities may be the result of synergistic effects of original flavonoids ,other metabo-lites and /or endogenous components .