CAJ | 학술논문

目的研究川芎嗪(TMP)在SAH后脑的保护作用。方法健康成年SD大鼠45只随机分为三组(n=15)。A为对照组；B为SAH组；C为SAH+TMP组。B、C两组均用自体动脉血两次注入于枕大池制作成SAH模型,C组用川芎嗪处理。各组均在第7d处死。结果在光镜下计数海马CA1区凋亡神经元,三组存在明显差异。免疫组化染色切片见bcl-2在C组较B组阳性细胞数多且强；而bax则相反。经过统计学处理分析后,各组神经元凋亡数目、Bcl-2、bax表达强度存在显著性差异(<0.05)。结论川芎嗪对SAH后对脑组织具有一定的保护作用。
목적연구천궁진(TMP)재SAH후뇌적보호작용。방법건강성년SD대서45지수궤분위삼조(n=15)。A위대조조；B위SAH조；C위SAH+TMP조。B、C량조균용자체동맥혈량차주입우침대지제작성SAH모형,C조용천궁진처리。각조균재제7d처사。결과재광경하계수해마CA1구조망신경원,삼조존재명현차이。면역조화염색절편견bcl-2재C조교B조양성세포수다차강；이bax칙상반。경과통계학처리분석후,각조신경원조망수목、Bcl-2、bax표체강도존재현저성차이(<0.05)。결론천궁진대SAH후대뇌조직구유일정적보호작용。
Objective To investigate the brain-protective effect of TMP after SAH in Rats. Methods 45 healthy adult SD rats were randomly divided into three groups (n=15). A is natural group,B into SAH group and C SAH+TMP group. B, C groups with autologous arterial blood into the cisterna magna made into SAH models. Group C with tetramethylpyrazine treatment. Al were executed in seventh days.Results Under light microscope counting neurons apoptosis in hippocampal CA1 region,there are obvious dif erences between the three groups.Immunohistochemical staining slice observation to see the BCL - 2 in group C than group B positive cells number and strong;while bax is the opposite. After statistical analysis, each neuron apoptosis number and intensity of the Bcl-2 and Bax expression exists significant dif erence ( <0.05). Conclusion TMP has the ef ect of brain-protective after SAH.