中国医药科学
中國醫藥科學
중국의약과학
CHINA MEDICINE AND PHARMACY
2013年
21期
57-58
,共2页
黄雨晴%卢小雪%张德威%黄锁义
黃雨晴%盧小雪%張德威%黃鎖義
황우청%로소설%장덕위%황쇄의
贵港功劳木%多糖%含量测定
貴港功勞木%多糖%含量測定
귀항공로목%다당%함량측정
Guigang mahonia%Polysaccharide%Content determination
目的:为了充分利用功劳木资源,为生产各种食品,医药品等多糖产品提供重要原料,提取功劳木中的多糖,并进行含量的测定。方法采用热水浸提法充分提取功劳木多糖,绘制葡萄糖标准曲线,利用Sevag 法脱蛋白提纯功劳木粗多糖并采用苯酚-硫酸法进行功劳木多糖含量的测定。结果测得样品中多糖的含量为0.689%,回收率为96.66%,RSD=0.158%(n=5)。结论功劳木中含有一定量的多糖,且该方法可作为检测贵港功劳木含量的较方便的方法。
目的:為瞭充分利用功勞木資源,為生產各種食品,醫藥品等多糖產品提供重要原料,提取功勞木中的多糖,併進行含量的測定。方法採用熱水浸提法充分提取功勞木多糖,繪製葡萄糖標準麯線,利用Sevag 法脫蛋白提純功勞木粗多糖併採用苯酚-硫痠法進行功勞木多糖含量的測定。結果測得樣品中多糖的含量為0.689%,迴收率為96.66%,RSD=0.158%(n=5)。結論功勞木中含有一定量的多糖,且該方法可作為檢測貴港功勞木含量的較方便的方法。
목적:위료충분이용공로목자원,위생산각충식품,의약품등다당산품제공중요원료,제취공로목중적다당,병진행함량적측정。방법채용열수침제법충분제취공로목다당,회제포도당표준곡선,이용Sevag 법탈단백제순공로목조다당병채용분분-류산법진행공로목다당함량적측정。결과측득양품중다당적함량위0.689%,회수솔위96.66%,RSD=0.158%(n=5)。결론공로목중함유일정량적다당,차해방법가작위검측귀항공로목함량적교방편적방법。
Objective In order to make full use of the resource of mahonia to provide important raw material for producing a variety of food products, pharmaceuticals and other polysaccharides products, and then it is necessary to study content determination of polysaccharides of mahonia. Methods With hot water to get the polysaccharides in mahonia as ample as possible, using glucose as standard substance and carrying the further preliminary purification by the method of Sevag at the same time with phenol-sulfuric acid method for content determination of polysaccharides of mahonia. Results The sample was measured the content of polysaccharide was 0.689%,the recovery rate was 96.66%,RSD=0.158% (n=5). Conclusion mahonia contains a certain polysaccharide,and it can be a convenient way to determine the content of polysaccharide in it.