中国医药科学
中國醫藥科學
중국의약과학
CHINA MEDICINE AND PHARMACY
2013年
21期
28-30
,共3页
小儿感冒片%质量标准%薄层色谱法%高效液相色谱法
小兒感冒片%質量標準%薄層色譜法%高效液相色譜法
소인감모편%질량표준%박층색보법%고효액상색보법
Pediatric cold tablets%Quality%Standards%TLC%HPLC
目的:建立人工牛黄的鉴别方法;建立用高效液相色谱法测定羌活、白芷中欧前胡素、异欧前胡素的含量测定方法。方法采用薄层色谱法鉴别人工牛黄,以胆酸、猪去氧胆酸、人工牛黄为对照物,点样量分别为10μL;采用C18色谱柱,乙腈-水(55∶45)为流动相;检测波长300nm。结果在紫外光灯(365nm)下检视样品斑点分离度较好,Rf值适宜;欧前胡素、异欧前胡素混合标准溶液进样量分别在0.029328~0.87984μg和0.024192~0.72576μg范围内,与峰面积呈良好的线性关系,欧前胡素平均回收率为99.51%,RDS=1.42%;异欧前胡素平均回收率为98.65%,RDS=2.66%。结论方法专属性强、耐用性高,准确度好,操作简便。
目的:建立人工牛黃的鑒彆方法;建立用高效液相色譜法測定羌活、白芷中歐前鬍素、異歐前鬍素的含量測定方法。方法採用薄層色譜法鑒彆人工牛黃,以膽痠、豬去氧膽痠、人工牛黃為對照物,點樣量分彆為10μL;採用C18色譜柱,乙腈-水(55∶45)為流動相;檢測波長300nm。結果在紫外光燈(365nm)下檢視樣品斑點分離度較好,Rf值適宜;歐前鬍素、異歐前鬍素混閤標準溶液進樣量分彆在0.029328~0.87984μg和0.024192~0.72576μg範圍內,與峰麵積呈良好的線性關繫,歐前鬍素平均迴收率為99.51%,RDS=1.42%;異歐前鬍素平均迴收率為98.65%,RDS=2.66%。結論方法專屬性彊、耐用性高,準確度好,操作簡便。
목적:건립인공우황적감별방법;건립용고효액상색보법측정강활、백지중구전호소、이구전호소적함량측정방법。방법채용박층색보법감별인공우황,이담산、저거양담산、인공우황위대조물,점양량분별위10μL;채용C18색보주,을정-수(55∶45)위류동상;검측파장300nm。결과재자외광등(365nm)하검시양품반점분리도교호,Rf치괄의;구전호소、이구전호소혼합표준용액진양량분별재0.029328~0.87984μg화0.024192~0.72576μg범위내,여봉면적정량호적선성관계,구전호소평균회수솔위99.51%,RDS=1.42%;이구전호소평균회수솔위98.65%,RDS=2.66%。결론방법전속성강、내용성고,준학도호,조작간편。
Objective To establish the method for identification of artificial bezoar; To establish a HPLC notopterygium, angelica imperatorin, isoimperatorin method for the determination. Methods Identification by TLC artificial bezoar to bile acids, hyodeoxycholic, artificial bezoar as a control substance, point injection volume was 10μL; The C18 Column, Acetonitrile- water (55 ︰ 45) as the mobile phase, detection wavelength 300nm. Results UV light (365nm), under review sample spots better separation, Rf value suitable; imperatorin, isoimperatorin mixed standard solution injection quantities were reported in 0.029 328-0.879 84μg and 0.024 192-0.725 76μg range, and the peak area was a good linear relationship, imperatorin the average recovery was 99.51%, RDS=1.42%;isoimperatorin the average recovery was 98.65%, RDS=2.66%. Conclusion This method is specific, high durability, good accuracy,and easy to operate.