中国基层医药
中國基層醫藥
중국기층의약
CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
2010年
3期
293-294
,共2页
胃肿瘤%胃炎,萎缩性%限制性内切酶置换基因%螺杆菌,幽门
胃腫瘤%胃炎,萎縮性%限製性內切酶置換基因%螺桿菌,幽門
위종류%위염,위축성%한제성내절매치환기인%라간균,유문
Stomach neoplasms%Gastritis,atrophic%Restriction endonudease-replacing gene%Helicobacter pylori
目的 探讨幽门螺杆菌(Hp)菌株中cagA和hrgA基因对胃癌、慢性萎缩性胃炎的致病作用及其检测的意义.方法 胃镜下诊断慢性胃炎的病例,钳取活体组织标本,组织学检查.胃癌及消化性溃疡术后切除标本,组织学检查确认,快速尿素酶法和聚合酶链反应(PCR)检测.结果 胃腺癌中18例hrgA阳性、6例hrgA阴性,20例cagA阳性、4例cagA阴性;消化性溃疡中6例hrgA阳性、8例hrgA阴性,12例cagA阳性、2例cagA阴性;慢性萎缩性胃炎中30例Hp阳性、8例Hp阴性,22例cagA阳性、16例cagA阴性;慢性浅表性胃炎中2例cagA阳性、20例cagA阴性.结论 Hp感染与胃癌、慢性萎缩性胄炎的发生有密切关系.PCR检测较快速尿素酶法准确.检测cngA和hrgA基因对了解Hp菌株的致病性、估计疾病程度、了解病变预后及临床治疗都具有重要意义.
目的 探討幽門螺桿菌(Hp)菌株中cagA和hrgA基因對胃癌、慢性萎縮性胃炎的緻病作用及其檢測的意義.方法 胃鏡下診斷慢性胃炎的病例,鉗取活體組織標本,組織學檢查.胃癌及消化性潰瘍術後切除標本,組織學檢查確認,快速尿素酶法和聚閤酶鏈反應(PCR)檢測.結果 胃腺癌中18例hrgA暘性、6例hrgA陰性,20例cagA暘性、4例cagA陰性;消化性潰瘍中6例hrgA暘性、8例hrgA陰性,12例cagA暘性、2例cagA陰性;慢性萎縮性胃炎中30例Hp暘性、8例Hp陰性,22例cagA暘性、16例cagA陰性;慢性淺錶性胃炎中2例cagA暘性、20例cagA陰性.結論 Hp感染與胃癌、慢性萎縮性冑炎的髮生有密切關繫.PCR檢測較快速尿素酶法準確.檢測cngA和hrgA基因對瞭解Hp菌株的緻病性、估計疾病程度、瞭解病變預後及臨床治療都具有重要意義.
목적 탐토유문라간균(Hp)균주중cagA화hrgA기인대위암、만성위축성위염적치병작용급기검측적의의.방법 위경하진단만성위염적병례,겸취활체조직표본,조직학검사.위암급소화성궤양술후절제표본,조직학검사학인,쾌속뇨소매법화취합매련반응(PCR)검측.결과 위선암중18례hrgA양성、6례hrgA음성,20례cagA양성、4례cagA음성;소화성궤양중6례hrgA양성、8례hrgA음성,12례cagA양성、2례cagA음성;만성위축성위염중30례Hp양성、8례Hp음성,22례cagA양성、16례cagA음성;만성천표성위염중2례cagA양성、20례cagA음성.결론 Hp감염여위암、만성위축성주염적발생유밀절관계.PCR검측교쾌속뇨소매법준학.검측cngA화hrgA기인대료해Hp균주적치병성、고계질병정도、료해병변예후급림상치료도구유중요의의.
Objective To investigate the role of hrgA and cagA genes of H.Pylori causing diseases and the value of detection in gastric carcinoma and atrophic gastritis.Methods Gastric biopsy specimens from 26 patients with gastric carcinoma and 14 with pepticulcer were obtained,and were performed histopathological examination,rapid urease test and polymerase chain reaction (PCR).Results Among the detected 26 gastric carcinoma cases,24 cases were gastric adenocarcinoma and 2 cases were gastric MALT lymphoma.Hp was present in 12 of 24 cases with gastric adenocarcinoma and in 12 of 14 cases with peptic ulcer by rapid urease test.Of the 24 cases with gastric adenocarcinoma,18 were hrgA positive and 20 were cagA positive;of the 14 cases with pepticulcer,6 were hrgA positive and 12 was cagA positive by PCR.Conclusions Hp could cause gastric carcinoma,and PCR was more reliable than the urease methods.To detect hrgA and cagA of Hp was very important for realizing the role of causing disease,assessing the damage,previewing the disease and choosing clinical treatment.
