CAJ | 학술논문

建立了同时测定牛奶中粘杆菌素和多粘菌素B素残留量的液相色谱-串联质谱(LC-MS/MS)的方法。样品通过5%高氯酸溶液沉淀蛋白，经乙醚除脂，Strata-X固相萃取柱净化，浓缩后经LC-MS/MS电喷雾正离子多反应检测模式(ESI+MRM)检测。结果显示，粘杆菌素和多粘菌素B在25~1000 ng/mL范围内均具有良好的线性关系，相关系数不低于0.9972，加标回收率为71.5%~98.1%，相对标准偏差为3.8%~9.5%。试验证明该方法前处理简单，快速，稳定性好，适用于大量样品中粘菌素和多粘菌素B残留的同时检测。
건립료동시측정우내중점간균소화다점균소B소잔류량적액상색보-천련질보(LC-MS/MS)적방법。양품통과5%고록산용액침정단백，경을미제지，Strata-X고상췌취주정화，농축후경LC-MS/MS전분무정리자다반응검측모식(ESI+MRM)검측。결과현시，점간균소화다점균소B재25~1000 ng/mL범위내균구유량호적선성관계，상관계수불저우0.9972，가표회수솔위71.5%~98.1%，상대표준편차위3.8%~9.5%。시험증명해방법전처리간단，쾌속，은정성호，괄용우대량양품중점균소화다점균소B잔류적동시검측。
A method was established to simultaneously detect colistinin and polymycin B in milk by liquid chromatography tandem mass spetrometry(LC-MS/MS).The samples,protein was precipitated with 5%perchloric acid and the fat was removed with diethyl ether,the other impurities were removed with Strata-X solid phase extraction cartridge,after concentration,theanalvtes were detected by LC-MS/MS(ESI)with multiple reaction monitoring(MRM).The results indicated that the calibration curves were in good linearity between the peak area and the concentration of 25~1 000 ng/mL for colistinin and polymycin B with correlation cofficients not less than 0.997 2,the spiked recoveries ranged from 71.5%~98.1%with reative standard deviations of 3.8%~9.5%.The proposed method was simple and rapid,the stability was good and it was suitable for the simultaneous quantitative detection of colistinin and polymycin B in milk.