CAJ | 학술논문

在建立大鼠原代肝细胞体外培养的基础上，研究了不同浓度的醋酸镉对肝细胞存活率的影响。在原代肝细胞体外培养的不同时间点(4、7、10、24 h)，用不同浓度的醋酸镉(0、0.5、1.0、2.0、4.0μmol/L)处理3 h，MTT法测定细胞的相对存活率。结果显示，随着镉剂量的增加，细胞的相对存活率降低。当醋酸镉浓度为4.0μmol/L，细胞的相对存活率为78.35%，与对照组有极显著性差异(P<0.01)；用4.0μmol/L醋酸镉在不同时间点处理肝细胞，与对照组相比，细胞存活率均极显著降低(P<0.01)。表明镉损害肝细胞具有一定的浓度依赖性。
재건립대서원대간세포체외배양적기출상，연구료불동농도적작산력대간세포존활솔적영향。재원대간세포체외배양적불동시간점(4、7、10、24 h)，용불동농도적작산력(0、0.5、1.0、2.0、4.0μmol/L)처리3 h，MTT법측정세포적상대존활솔。결과현시，수착력제량적증가，세포적상대존활솔강저。당작산력농도위4.0μmol/L，세포적상대존활솔위78.35%，여대조조유겁현저성차이(P<0.01)；용4.0μmol/L작산력재불동시간점처리간세포，여대조조상비，세포존활솔균겁현저강저(P<0.01)。표명력손해간세포구유일정적농도의뢰성。
In this study,the rat primary hepatocyte culture system in vitro was used to study the changes of cell viability in different concentrations of cadmium . In primary hepatocyte cultured at different time points in vitro(4 h、7 h、10 h and 24 h),which were incubated with cadmium acetate of different concentrations(0μmol/L,0.5μmol/L,1.0μmol/L,2.0μmol/L,4.0μmol/L)for 3 h.The results of MTT assay showed that Cd reduced the motility rat of primary hepatocytes at dose-dependant. After exposed to 4.0 μmol/L cadmium,the cell motility decreased to 78.35%,showing a significantly difference from the control group(P<0.01).Hepatocytes were cultured for 4 h,7 h and 24 h and treated with 4.0μmol/L Cd for 3 h in vitro,cell viability was significantly decreased(P<0.01). The results suggested that Cd-induced cell viability was at a concentration-dependent manner,and the cell initial state could significantly affect Cd-induced cell viability.