CAJ | 학술논문

利用RT-PCR技术从山荆子cDNA中克隆CBF基因，ORF全长756 bp，编码252个氨基酸；其氨基酸序列含典型的CBF基因蛋白保守的序列AP2/EREB DNA结合域及CBF家族蛋白特征短多肽序列(PKK/RPAGRxKFxETRHP和DSAWR)；与Genebank上收录的几种植物CBF基因进行氨基酸同源性比对，结果发现山荆子CBF转录因子MbCBF和苹果CBF/DREB1基因的氨基酸序列相似性为98%；与梅树CBF相似性为67%；进一步构建了植物表达载体，为利用CBF基因提高植物抗逆性奠定基础。
이용RT-PCR기술종산형자cDNA중극륭CBF기인，ORF전장756 bp，편마252개안기산；기안기산서렬함전형적CBF기인단백보수적서렬AP2/EREB DNA결합역급CBF가족단백특정단다태서렬(PKK/RPAGRxKFxETRHP화DSAWR)；여Genebank상수록적궤충식물CBF기인진행안기산동원성비대，결과발현산형자CBF전록인자MbCBF화평과CBF/DREB1기인적안기산서렬상사성위98%；여매수CBF상사성위67%；진일보구건료식물표체재체，위이용CBF기인제고식물항역성전정기출。
The CBF transcription factors were cloned by RT-PCR technique from the plants of Malus baccata.The full-length of open reading frame (ORF) was 756 bp,encoding 252 amino acids;The amino acids sequence owned the characteristics of CBF protein,which contained an AP2/EREB DNA binding domain and two special short amino acids sequences;The amino acids sequences of MbCBF had highly homologous with CBF genes of other plants included in Genebank,reached 98%with Malus domestica,and 67%with Prunus mume respectively;Then we connected the gene into the plant expression vector PBI121,which laid the foundation for the utilization of CBF genes to improve plant stress tolerance.