CAJ | 학술논문

为获得在无核荔枝中胚珠退化过程中差异表达全长MY B转录因子基因，并为其功能研究打下基础，根据从已构建的无核荔枝胚珠败育SSH消减文库中得到的差异表达的MY B转录因子EST序列，提取高质量的总RNA，运用SMART RACE技术，获得一个1177bp的MY B基因核苷酸序列。生物信息学分析表明，该序列含有879 bp的开放阅读框，推测的蛋白质为292个氨基酸，具有2个SANT结构功能域，在系统发育树上与大豆MY B基因亲缘关系最近。该基因为MY B基因家族中的新成员。
위획득재무핵려지중배주퇴화과정중차이표체전장MY B전록인자기인，병위기공능연구타하기출，근거종이구건적무핵려지배주패육SSH소감문고중득도적차이표체적MY B전록인자EST서렬，제취고질량적총RNA，운용SMART RACE기술，획득일개1177bp적MY B기인핵감산서렬。생물신식학분석표명，해서렬함유879 bp적개방열독광，추측적단백질위292개안기산，구유2개SANT결구공능역，재계통발육수상여대두MY B기인친연관계최근。해기인위MY B기인가족중적신성원。
To obtain the full length of MY B gene and lay the foundation its function research that was differentially expressed in ovule degeneration of seedless litchi. The EST encoding MY B was obtained from young abortive ovule of seedless litchi via SSH(suppression subtractive hybridization),high quality total RNA was extracted and obtained a 1 177 bp full length cDNA sequence by SMAT-RACE method,bioinformatics analysis showed that it included an 879 bp open reading frame,encoded a deduced protein with 292 amino acids,and contained 2 SANT domains,phylogenetic tree showed it got the closest relatives with Glycine max. The results showed that it's a new member of the MY B family.