CAJ | 학술논문

为研究生肌因子如Myogenin和Myf5在调控肌特异基因表达时的作用机制，将这2个因子共转染非肌细胞，为确保转染效果，依次将这2个因子的编码序列克隆在双表达载体pVITRO2上。测序结果显示，克隆到载体的myogenin和Myf5编码区序列正确；初步的功能检测表明，它们都能有效地激活各自的靶基因。
위연구생기인자여Myogenin화Myf5재조공기특이기인표체시적작용궤제，장저2개인자공전염비기세포，위학보전염효과，의차장저2개인자적편마서렬극륭재쌍표체재체pVITRO2상。측서결과현시，극륭도재체적myogenin화Myf5편마구서렬정학；초보적공능검측표명，타문도능유효지격활각자적파기인。
Muscle development was specially controlled by the myogenic factors such as myogenin and Myf5 . It is necessary to cotransfect these two myogenic factors into non-muscle cells for further investigating the machanism by which they regulate the muscle specific genes. To gain high transfection efficiency,the coding sequences of myo-genin and Myf5 were cloned successively into the pVITRO2 , an expression vector containing two multiple cloning sites ( MCS) for the convenient cloning of two cDNAs. Sequencing results proved that the cloned myogenin and Myf5 are correct,and the transfection assay suggested they could activate their respective target genes respectively.