CAJ | 학술논문

目的：研究中国药品检验用玫瑰红钠琼脂培养基整体质量水平,为《中国药典》等药品质量标准的修订提供技术支持。方法：使用4个质控菌对国内外7家培养基生产商的玫瑰红钠琼脂培养基进行质量评价。结果：不同培养基的pH存在差异。7个生产商的培养基对白色念珠菌和黑曲霉的生长率均大于0.7,生长率均值差异无统计学意义(P>0.05)。7个培养基和对照培养基对金黄色葡萄球菌的选择因子均大于6；8个培养基产品对大肠埃希菌的选择因子表现出差别,A、B、C、E、G和对照培养基无抑制性(选择性)；D和F表现出一定选择性。结论：不同厂家的药品检验用玫瑰红钠琼脂培养基的促生长能力表现一致；不同厂家产品的pH和对大肠埃希菌的选择性存在差异。
목적：연구중국약품검험용매괴홍납경지배양기정체질량수평,위《중국약전》등약품질량표준적수정제공기술지지。방법：사용4개질공균대국내외7가배양기생산상적매괴홍납경지배양기진행질량평개。결과：불동배양기적pH존재차이。7개생산상적배양기대백색념주균화흑곡매적생장솔균대우0.7,생장솔균치차이무통계학의의(P>0.05)。7개배양기화대조배양기대금황색포도구균적선택인자균대우6；8개배양기산품대대장애희균적선택인자표현출차별,A、B、C、E、G화대조배양기무억제성(선택성)；D화F표현출일정선택성。결론：불동엄가적약품검험용매괴홍납경지배양기적촉생장능력표현일치；불동엄가산품적pH화대대장애희균적선택성존재차이。
Objective:To investigate overall quality status of rose bengal agar culture medium for drug control in China to provide technical support for the drug standard revision. Methods:Four quality control microorganisms, namely Aspergillus niger, Candida al-bicans, Staphylococcus aureus and Escherichia coli, were used to evaluate the quality of rose bengal agar culture medium from 7 domes-tic and foreign manufacturers. Results:Including the control medium, the pH value of all the 8 media was different. The growth rate of Aspergillus niger and Candida albicans in the seven tested media were all above 0. 7 without significant difference (P>0. 05). The selectivity factors of Staphylococcus aureus in the seven media and the control medium was more than 6. The eight media showed differ-ences in the selectivity factors of Escherichia coli. Among them, A, B, C, E, G and the control medium had no inhibitory ( selectivi-ty) , while D and F showed certain selectivity. Conclusion:The rose bengal agar media from different manufacturers for drug control show similar growth-promoting ability with different pH value and selectivity to Escherichia coli.