中国药师
中國藥師
중국약사
CHINA PHARMACIST
2014年
5期
871-872
,共2页
高效液相色谱法%寮刁竹%丹皮酚
高效液相色譜法%寮刁竹%丹皮酚
고효액상색보법%료조죽%단피분
HPLC%Cynanchum paniculatum%Paeonol
目的：对不同产地的寮刁竹药材中丹皮酚含量进行测定,为更好地评价寮刁竹药材的质量提供依据。方法：用高效液相色谱法采用Promosil C18色谱柱(200 mm ×4.6 mm,5μm),以甲醇-水(55∶45)为流动相,流速1.0 ml·min-1,检测波长为274 nm,进样量为10μl。结果：丹皮酚在0.0494~0.7908 mg·ml-1范围内线性关系良好(r=0.9999),回收率为99.98%, RSD为0.17%,不同产地寮刁竹中丹皮酚含量有明显差异。结论：该方法简便、准确、重复性好,可用于不同产地寮刁竹药材中丹皮酚含量的测定。
目的：對不同產地的寮刁竹藥材中丹皮酚含量進行測定,為更好地評價寮刁竹藥材的質量提供依據。方法：用高效液相色譜法採用Promosil C18色譜柱(200 mm ×4.6 mm,5μm),以甲醇-水(55∶45)為流動相,流速1.0 ml·min-1,檢測波長為274 nm,進樣量為10μl。結果：丹皮酚在0.0494~0.7908 mg·ml-1範圍內線性關繫良好(r=0.9999),迴收率為99.98%, RSD為0.17%,不同產地寮刁竹中丹皮酚含量有明顯差異。結論：該方法簡便、準確、重複性好,可用于不同產地寮刁竹藥材中丹皮酚含量的測定。
목적：대불동산지적료조죽약재중단피분함량진행측정,위경호지평개료조죽약재적질량제공의거。방법：용고효액상색보법채용Promosil C18색보주(200 mm ×4.6 mm,5μm),이갑순-수(55∶45)위류동상,류속1.0 ml·min-1,검측파장위274 nm,진양량위10μl。결과：단피분재0.0494~0.7908 mg·ml-1범위내선성관계량호(r=0.9999),회수솔위99.98%, RSD위0.17%,불동산지료조죽중단피분함량유명현차이。결론：해방법간편、준학、중복성호,가용우불동산지료조죽약재중단피분함량적측정。
Objective:To determine paeonol in Cynanchum paniculatum from different habitats by HPLC to provide better quality evaluation for Cynanchum paniculatum. Methods:A Promosil C18 column (200 mm × 4. 6 mm, 5 μm) was adopted, methanol-water (55∶45) was used as the mobile phase, the flow rate was 1. 0 ml·min-1 , the detection wavelength was at 274 nm, and the injection volume was 10μl. Results:Paeonol showed a good linearity(r=0. 999 9) within the range of 0. 049 4-0. 790 8 mg·ml-1,and the re-covery was 99. 98% with RSD of 0. 17%. The content of paeonol in Cynanchum paniculatum from different habitats showed significant difference. Conclusion:The method is simple, accurate and reproducible, which can be used to determine the content of paeonol in Cynanchum paniculatum from different habitats.
