CAJ | 학술논문

目的：在细胞水平研究绞股蓝皂甙抗氧化损伤的影响及机制。方法原代培养并建立人衰老皮肤成纤维细胞系，0、5、10、15μg/ml绞股蓝皂甙处理细胞，24、72、96h后，MTT检测细胞增殖；72h后检测SOD 活性、MDA 含量。结果绞股蓝皂甙可以促进人衰老成纤维细胞增殖，增强细胞SOD活性，降低MDA含量，且呈浓度依赖。结论绞股蓝皂甙可以通过提高SOD活性，降低MDA含量，削弱氧化应激反应对细胞的损伤，使细胞增殖能力增强，延缓细胞衰老。
목적：재세포수평연구교고람조대항양화손상적영향급궤제。방법원대배양병건립인쇠로피부성섬유세포계，0、5、10、15μg/ml교고람조대처리세포，24、72、96h후，MTT검측세포증식；72h후검측SOD 활성、MDA 함량。결과교고람조대가이촉진인쇠로성섬유세포증식，증강세포SOD활성，강저MDA함량，차정농도의뢰。결론교고람조대가이통과제고SOD활성，강저MDA함량，삭약양화응격반응대세포적손상，사세포증식능력증강，연완세포쇠로。
Objective To study the antioxidative damage ef ect and mechanism of Gypenosides at the cellular level. Methods Fibroblasts were cultured and fed by 0μg/ml,5μg/ ml,10μg/ ml and 15μg/ ml Gypenosides for 24h,72h and 96h. MTT method tested the cellproliferation. Relative level of MDA and activity of SOD in cells were measured. Results Gypenosides could promote aged fibroblasts proliferation. At the same time, decreased MDA volume and increased the activity of SOD. Conclusions Gypenosides protected the aged fibroblasts in oxidative stress and therefore could delay the cells ageing.