中南民族大学学报(自然科学版)
中南民族大學學報(自然科學版)
중남민족대학학보(자연과학판)
JOURNAL OF SOUTH-CENTRAL UNIVERSITY FOR NATIONALITIES(NATURAL SCIENCE EDITION)
2014年
3期
37-40
,共4页
李劲%李可可%姚品芳%陈静波%陈真
李勁%李可可%姚品芳%陳靜波%陳真
리경%리가가%요품방%진정파%진진
MMP3基因SNP%河南不同人群%基因分型%PCR-RFLP%logitic回归分析
MMP3基因SNP%河南不同人群%基因分型%PCR-RFLP%logitic迴歸分析
MMP3기인SNP%하남불동인군%기인분형%PCR-RFLP%logitic회귀분석
MMP3%SNP%genotyping%ESCC%different groups of Henan people
为研究MMP3基因5′端转录调控区rs102715950(5A/6A)单核苷酸多态性与河南人群食管癌易感性的关系,通过蛋白酶K法提取基因组DNA,Nested PCR扩增目的基因片段,用Tth111I进行酶切,PAGE电泳检测酶切结果,对河南散发、正常、高发区3种人群进行了MMP3基因SNP rs102715950(5A/6A)基因分型,用SPSS软件作了Logistic回归统计分析.结果表明:在MMP3基因rs102715950(5A/6A)单核苷酸多态性上,河南食管癌散发人群、河南正常人群、湖北的河南移民人群3类人群相互之间并无显著性差异( p>0.01),说明MMP3基因rs102715950(5A/6A)单核苷酸多态性与河南人群的食管鳞癌易感性无显著相关性.
為研究MMP3基因5′耑轉錄調控區rs102715950(5A/6A)單覈苷痠多態性與河南人群食管癌易感性的關繫,通過蛋白酶K法提取基因組DNA,Nested PCR擴增目的基因片段,用Tth111I進行酶切,PAGE電泳檢測酶切結果,對河南散髮、正常、高髮區3種人群進行瞭MMP3基因SNP rs102715950(5A/6A)基因分型,用SPSS軟件作瞭Logistic迴歸統計分析.結果錶明:在MMP3基因rs102715950(5A/6A)單覈苷痠多態性上,河南食管癌散髮人群、河南正常人群、湖北的河南移民人群3類人群相互之間併無顯著性差異( p>0.01),說明MMP3基因rs102715950(5A/6A)單覈苷痠多態性與河南人群的食管鱗癌易感性無顯著相關性.
위연구MMP3기인5′단전록조공구rs102715950(5A/6A)단핵감산다태성여하남인군식관암역감성적관계,통과단백매K법제취기인조DNA,Nested PCR확증목적기인편단,용Tth111I진행매절,PAGE전영검측매절결과,대하남산발、정상、고발구3충인군진행료MMP3기인SNP rs102715950(5A/6A)기인분형,용SPSS연건작료Logistic회귀통계분석.결과표명:재MMP3기인rs102715950(5A/6A)단핵감산다태성상,하남식관암산발인군、하남정상인군、호북적하남이민인군3류인군상호지간병무현저성차이( p>0.01),설명MMP3기인rs102715950(5A/6A)단핵감산다태성여하남인군적식관린암역감성무현저상관성.
To explore the association between SNP rs 102715950(5A/6A) in 5′-end regulation region of MMP3 gene and susceptibility to esophageal squamous cell carcinoma ( ESCC ) in different groups of people in Henan , Genomic DNA was extracted from peripheral blood with the proteinase K method .The genotyping was carried out by PCR-RFLP and analyzed comparatively.Then the results of genotyping were analyzed by SPSS 19.0.The results showed that there was no statistical differences in the genotyping of MMP3 SNP rs102715950(5A/6A) of among 3 groups of people ,including sporadic cases of esophageal cancer , migrants of the high incidence area and healthy controls in Henan .So there was no obvious correlations between the MMP3 SNP rs102715950(5A/6A) with the susceptibility of esophageal squamous cell carcinoma among the people in Henan .