CAJ | 학술논문

为研究MMP3基因5′端转录调控区rs102715950（5A/6A）单核苷酸多态性与河南人群食管癌易感性的关系，通过蛋白酶K法提取基因组DNA，Nested PCR扩增目的基因片段，用Tth111I进行酶切，PAGE电泳检测酶切结果，对河南散发、正常、高发区3种人群进行了MMP3基因SNP rs102715950（5A/6A）基因分型，用SPSS软件作了Logistic回归统计分析．结果表明：在MMP3基因rs102715950（5A/6A）单核苷酸多态性上，河南食管癌散发人群、河南正常人群、湖北的河南移民人群3类人群相互之间并无显著性差异（ p＞0．01），说明MMP3基因rs102715950（5A/6A）单核苷酸多态性与河南人群的食管鳞癌易感性无显著相关性．
위연구MMP3기인5′단전록조공구rs102715950（5A/6A）단핵감산다태성여하남인군식관암역감성적관계，통과단백매K법제취기인조DNA，Nested PCR확증목적기인편단，용Tth111I진행매절，PAGE전영검측매절결과，대하남산발、정상、고발구3충인군진행료MMP3기인SNP rs102715950（5A/6A）기인분형，용SPSS연건작료Logistic회귀통계분석．결과표명：재MMP3기인rs102715950（5A/6A）단핵감산다태성상，하남식관암산발인군、하남정상인군、호북적하남이민인군3류인군상호지간병무현저성차이（ p＞0．01），설명MMP3기인rs102715950（5A/6A）단핵감산다태성여하남인군적식관린암역감성무현저상관성．
To explore the association between SNP rs 102715950(5A/6A) in 5′-end regulation region of MMP3 gene and susceptibility to esophageal squamous cell carcinoma ( ESCC ) in different groups of people in Henan , Genomic DNA was extracted from peripheral blood with the proteinase K method .The genotyping was carried out by PCR-RFLP and analyzed comparatively.Then the results of genotyping were analyzed by SPSS 19.0.The results showed that there was no statistical differences in the genotyping of MMP3 SNP rs102715950(5A/6A) of among 3 groups of people ,including sporadic cases of esophageal cancer , migrants of the high incidence area and healthy controls in Henan .So there was no obvious correlations between the MMP3 SNP rs102715950(5A/6A) with the susceptibility of esophageal squamous cell carcinoma among the people in Henan .