CAJ | 학술논문

为探讨铁棒锤的毒效关系，提取分离其氯仿部位并采用HPLC分析其主成分，比较了该部位与铁棒锤其他活性部位的急性毒性，以热板法和扭体法考察了不同剂量铁棒锤氯仿部位对小鼠的镇痛作用．结果表明：铁棒锤不同部位对小鼠急性毒性强度差别较大，氯仿部位的毒性最强，正丁醇和石油醚部位次之，三者的LD50分别为37．514，6766．928，5492．337 mg/kg．不同剂量的铁棒锤氯仿部位均能提高小鼠热板痛阈值，呈剂量依赖性关系．20 mg/kg铁棒锤60 min时的镇痛百分率为108．7％，远低于阳性对照10 mg/kg吗啡组（261．6％），但高于同剂量的铁棒锤总提物组（75．2％）．不同剂量的铁棒锤氯仿部位均能有效抑制冰醋酸对所致小鼠扭体反应，呈剂量依赖性关系．20 mg/kg组的扭体抑制率为76．4％，明显高于200 mg/kg阿司匹林（50．5％）和20 mg/kg总提物组（51．0％）．说明铁棒锤氯仿部位具有显著的毒性和镇痛活性呈剂量依赖性，推测其镇痛活性与毒性存在一定的关联．
위탐토철봉추적독효관계，제취분리기록방부위병채용HPLC분석기주성분，비교료해부위여철봉추기타활성부위적급성독성，이열판법화뉴체법고찰료불동제량철봉추록방부위대소서적진통작용．결과표명：철봉추불동부위대소서급성독성강도차별교대，록방부위적독성최강，정정순화석유미부위차지，삼자적LD50분별위37．514，6766．928，5492．337 mg/kg．불동제량적철봉추록방부위균능제고소서열판통역치，정제량의뢰성관계．20 mg/kg철봉추60 min시적진통백분솔위108．7％，원저우양성대조10 mg/kg마배조（261．6％），단고우동제량적철봉추총제물조（75．2％）．불동제량적철봉추록방부위균능유효억제빙작산대소치소서뉴체반응，정제량의뢰성관계．20 mg/kg조적뉴체억제솔위76．4％，명현고우200 mg/kg아사필림（50．5％）화20 mg/kg총제물조（51．0％）．설명철봉추록방부위구유현저적독성화진통활성정제량의뢰성，추측기진통활성여독성존재일정적관련．
To study the toxicity of Aconitum Pendulum Busch.its chloroform extract was separated and analyzed by HPLC . An acute toxicity test was used to compare the toxicity of different extracts , then hot-plate test and acid-induced writhing test were used to assess the analgesic effect of the 20, 10, and 5 mg/kg chloroform extracts.The results indicated that the toxicity varied among different extracts .The most toxic extract was that of chloroform , followed by n-butyl alcohol and petroleum ether, of which the LD50 was 37.514, 6766.928, 5492.337 mg/kg respectively.Different doses of chloroform extract could all increase the pain threshold in a dose-dependent manner in hot plate test .The analgesia percentage of the 20 mg/kg chloroform extract was 108.7%in 60 minutes, far less than that of the 10 mg/kg morphine (261.6%), but was larger than that of the total extract ( 75.2%) .Different doses of chloroform extract could inhibit acetic acid induced writhing response in a dose-dependent manner .The writhing inhibition rate of the 20 mg/kg chloroform extract was 76.4%, significantly higher than that of the 200 mg/kg aspirin (50.5%) and the 20 mg/kg total extract (51.0%).The chloroform extract exhibited significant toxicity and analgesic activity in a dose-dependent manner , and there is probably some correlation between the analgesic activity and toxicity .