中国实用医药
中國實用醫藥
중국실용의약
CHINA PRACTICAL MEDICAL
2013年
33期
253-254
,共2页
E3泛素连接酶%卵巢癌%真核表达质粒
E3汎素連接酶%卵巢癌%真覈錶達質粒
E3범소련접매%란소암%진핵표체질립
Homologous to the E6-associated protein carboxyl terminus domain Containing 3(HETCD3)%Ovarian cancer%Eukaryotic expression plasmid
目的：克隆E3泛素连接酶(homologous to the E6-associated protein carboxyl terminus domain containing 3, HECTD3)基因及构建真核表达质粒。方法提取人卵巢癌细胞SKOV-3细胞RNA,并逆转录为cDNA,以此作为模板PCR法扩增HECTD3基因,将其克隆至真核表达载体pcDNA3.1(+),测序验证构建质粒中包含HECTD3基因。结论 HECTD3基因克隆及真核表达质粒构建成功,可以用于后续卵巢癌发生发展的分子生物学研究。
目的：剋隆E3汎素連接酶(homologous to the E6-associated protein carboxyl terminus domain containing 3, HECTD3)基因及構建真覈錶達質粒。方法提取人卵巢癌細胞SKOV-3細胞RNA,併逆轉錄為cDNA,以此作為模闆PCR法擴增HECTD3基因,將其剋隆至真覈錶達載體pcDNA3.1(+),測序驗證構建質粒中包含HECTD3基因。結論 HECTD3基因剋隆及真覈錶達質粒構建成功,可以用于後續卵巢癌髮生髮展的分子生物學研究。
목적：극륭E3범소련접매(homologous to the E6-associated protein carboxyl terminus domain containing 3, HECTD3)기인급구건진핵표체질립。방법제취인란소암세포SKOV-3세포RNA,병역전록위cDNA,이차작위모판PCR법확증HECTD3기인,장기극륭지진핵표체재체pcDNA3.1(+),측서험증구건질립중포함HECTD3기인。결론 HECTD3기인극륭급진핵표체질립구건성공,가이용우후속란소암발생발전적분자생물학연구。
Objective To clone the gene of homologous to the E6-associated protein carboxyl terminus domain containing 3 (HECTD3) and construct the HECTD3 gene expression plasmid. Methods The HECTD3 gene was cloned from ovarian cell cDNA by PCR and recombined into the eukaryotic expression plasmid pcDNA3.1(+). Results the HECTD3 gene was confirmed in the eukaryotic expression plasmid by sequence analysis. Conclusion The HECTD3 gene expression plasmid can be the tools for further research of ovarian cancer.
