CAJ | 학술논문

　　为探究谷子抗病育种的理论基础，采用Blast比对的方法，利用已知的谷子抗“拿捕净”除草剂的基因组序列，对谷子抗“拿捕净”基因进行SSR分子连锁标记筛选研究。利用抗“拿捕净”基因的已知序列找到了在谷子基因组中第7和第9条染色体上均存在同源序列，分别在这些同源序列上下游寻找SSR引物，在谷子F2群体中进行SSR标记的筛选，进行共分离分析。结果表明：位于第7染色体上的2个SSR标记SIMS13569和SIMS13512与谷子抗“拿捕净”基因性状共分离，即表现为连锁遗传，该标记可用于分子标记辅助选择，对谷子抗“拿捕净”基因育种具有重要的现实意义；而第9染色体上的序列不具有抗“拿捕净”的作用。
　　위탐구곡자항병육충적이론기출，채용Blast비대적방법，이용이지적곡자항“나포정”제초제적기인조서렬，대곡자항“나포정”기인진행SSR분자련쇄표기사선연구。이용항“나포정”기인적이지서렬조도료재곡자기인조중제7화제9조염색체상균존재동원서렬，분별재저사동원서렬상하유심조SSR인물，재곡자F2군체중진행SSR표기적사선，진행공분리분석。결과표명：위우제7염색체상적2개SSR표기SIMS13569화SIMS13512여곡자항“나포정”기인성상공분리，즉표현위련쇄유전，해표기가용우분자표기보조선택，대곡자항“나포정”기인육충구유중요적현실의의；이제9염색체상적서렬불구유항“나포정”적작용。
Utilizing the known genome sequence of herbicide-resistant to Sethoxydim in foxtail millet ,selection SSR markers which was linkage of herbicide-resistant to Sethoxydim was studied .Using Blast alignment meth-od ,the homologous sequences of herbicide-resistant to Sethoxydim gene was found in the seventh and the ninth chromosomes in foxtail millet .In the upstream and downstream of homologous sequences ,primers were de-signed to select SSR molecular markers .In the F2 population of foxtail millet ,the herbicide-resistant to Se-thoxydim gene was linkage of two SSR markers ,SIMS13569 and SIMS13512 which were in the seventh chro-mosome ,and could be used for molecular marker assisted selection .It is important to the genetic breeding of herbicide-resistant to Sethoxydim in foxtail millet .However ,the homologous sequence in the ninth chromosome was not own the effect of herbicide-resistant to Sethoxydim .