安徽医学
安徽醫學
안휘의학
ANHUI MEDICAL JOURNAL
2014年
3期
272-274
,共3页
内皮祖细胞%聚乳酸-聚乙醇酸%糖尿病%血管化
內皮祖細胞%聚乳痠-聚乙醇痠%糖尿病%血管化
내피조세포%취유산-취을순산%당뇨병%혈관화
Endothelial progenitor cells%Poly(lactic-co-glycolic acid)%Diabetes%Vascularization
目的:体外培养兔内皮祖细胞(EPCs)并种植于聚乳酸-聚乙醇酸(PLGA)上培养形成移植物,植入兔体内,观察其血管化的程度,探讨其促进糖尿病创面血管化的可行性。方法体外分离、培养、鉴定兔EPCs,并将其种植于PLGA上,形成细胞-支架复合移植物;造兔糖尿病模型,植入复合移植物的为实验组,仅植入支架材料的为对照组,分别于4周、8周后处死动物,复合移植物行组织学及免疫组化检测,观察血管化程度。结果术后8周,实验组移植物生长良好,可见较多的功能性新生血管穿插长入其中,对照组未见明显的血管生成(P<0.05)。结论 EPCs参与组织修复及促进新生血管生成,为糖尿病创面的愈合提供了血管化的基础。
目的:體外培養兔內皮祖細胞(EPCs)併種植于聚乳痠-聚乙醇痠(PLGA)上培養形成移植物,植入兔體內,觀察其血管化的程度,探討其促進糖尿病創麵血管化的可行性。方法體外分離、培養、鑒定兔EPCs,併將其種植于PLGA上,形成細胞-支架複閤移植物;造兔糖尿病模型,植入複閤移植物的為實驗組,僅植入支架材料的為對照組,分彆于4週、8週後處死動物,複閤移植物行組織學及免疫組化檢測,觀察血管化程度。結果術後8週,實驗組移植物生長良好,可見較多的功能性新生血管穿插長入其中,對照組未見明顯的血管生成(P<0.05)。結論 EPCs參與組織脩複及促進新生血管生成,為糖尿病創麵的愈閤提供瞭血管化的基礎。
목적:체외배양토내피조세포(EPCs)병충식우취유산-취을순산(PLGA)상배양형성이식물,식입토체내,관찰기혈관화적정도,탐토기촉진당뇨병창면혈관화적가행성。방법체외분리、배양、감정토EPCs,병장기충식우PLGA상,형성세포-지가복합이식물;조토당뇨병모형,식입복합이식물적위실험조,부식입지가재료적위대조조,분별우4주、8주후처사동물,복합이식물행조직학급면역조화검측,관찰혈관화정도。결과술후8주,실험조이식물생장량호,가견교다적공능성신생혈관천삽장입기중,대조조미견명현적혈관생성(P<0.05)。결론 EPCs삼여조직수복급촉진신생혈관생성,위당뇨병창면적유합제공료혈관화적기출。
Objective Rabbit EPCs were cultured in vitro and planted on PLGA to form the grafts,which were then implanted in rabbits to observe the degree of vascularization,which may promote the cure of diabetic vascular wound.Methods In vitro,we isolated,cul-tured,identified the rabbit EPCs,and planted these cells on the PLGA to form cell-scaffold grafts;then diabetic rabbit model was made,and composite graft was implanted in the experimental group,only the scaffold ones for the experimental group.Respectively,after 4 and 8 weeks, the animals were sacrificed;in order to observe the vascularization of complex,the composite graft was detected by histological and immuno-histochemical methods.Results After 8 weeks,the experimental group graft material grew well,which were seen more functional neovascular-ization interspersed among it and in the control group,the scar contracture occurred with no significant angiogenesis.Conclusion EPCs are involved in tissue repair and promote angiogenesis,which provides a vascularized foundation for the healing of diabetes wound.