CAJ | 학술논문

目的：探讨灵芝多糖联合二甲双胍对2型糖尿病大鼠心肌纤维化的预防作用及其作用机制。方法 SD大鼠高脂饮食喂养4周后,腹腔注射链脲佐菌素(STZ)30 mg·kg-1建立2型糖尿病(type 2 diabetes mellitus,T2DM)模型。成模后将大鼠随机分为正常对照组、模型组、灵芝多糖组(灵芝多糖600 mg·kg-1)、二甲双胍组(二甲双胍600 mg·kg-1)及联合用药组(灵芝多糖300 mg · kg-1+二甲双胍300 mg · kg-1)。药物治疗12周末测量大鼠空腹血糖；饱和苦味酸-天狼猩红染色下观察心肌纤维化程度；荧光分光光度计法检测大鼠血清晚期糖基化终末产物( advanced glycosylation end products,AGEs)的含量；测量心肌过氧化氢酶( CAT)、谷胱甘肽过氧化物酶( GSH-Px )水平；免疫组化法和蛋白印迹法检测心肌组织AGEs及CTGF蛋白的表达。结果联合用药组可有效抑制心肌纤维化的病理进程,升高心肌组织CAT、GSH-Px水平及降低血清AGEs含量,减少心肌组织AGEs及CTGF的表达。结论灵芝多糖联合二甲双胍可能通过抑制心肌氧化应激,降低血清AGEs水平,以及下调心肌AGEs、CTGF的表达来预防心肌纤维化。
목적：탐토령지다당연합이갑쌍고대2형당뇨병대서심기섬유화적예방작용급기작용궤제。방법 SD대서고지음식위양4주후,복강주사련뇨좌균소(STZ)30 mg·kg-1건립2형당뇨병(type 2 diabetes mellitus,T2DM)모형。성모후장대서수궤분위정상대조조、모형조、령지다당조(령지다당600 mg·kg-1)、이갑쌍고조(이갑쌍고600 mg·kg-1)급연합용약조(령지다당300 mg · kg-1+이갑쌍고300 mg · kg-1)。약물치료12주말측량대서공복혈당；포화고미산-천랑성홍염색하관찰심기섬유화정도；형광분광광도계법검측대서혈청만기당기화종말산물( advanced glycosylation end products,AGEs)적함량；측량심기과양화경매( CAT)、곡광감태과양화물매( GSH-Px )수평；면역조화법화단백인적법검측심기조직AGEs급CTGF단백적표체。결과연합용약조가유효억제심기섬유화적병리진정,승고심기조직CAT、GSH-Px수평급강저혈청AGEs함량,감소심기조직AGEs급CTGF적표체。결론령지다당연합이갑쌍고가능통과억제심기양화응격,강저혈청AGEs수평,이급하조심기AGEs、CTGF적표체래예방심기섬유화。
Aim To study the effects of ganoderma lu-cidum polysaccharides and metformin on myocardial fi-brosis of type 2 diabetic rats and its mechanism. Methods SD rats were fed with high fat diet for 4 weeks, and then were injected with streptozotocin (30mg·kg-1 ) to replicate type 2 diabetic model. The diabetic rats were randomized into normal control group,diabetes group, ganoderma lucidum polysaccha-rides group ( 600 mg · kg-1 ) , metformin group ( 600 mg·kg-1 ) , and combination group( ganoderma lucid-um polysaccharides 300 mg·kg-1 +metformin 300 mg ·kg-1 ) . After 12 weeks’ treatment,the levels of fast-ing serum glucose were determined and the extent of myocardial fibrosis was observed by Picro-sirius red staining. The contents of AGEs in serum were deter-mined by fluorescence spectrophotometer. The activities of CAT and GSH-Px in myocardium were detected. Im-munohistochemical method and Western blot were used to detect myocardial tissue AGEs and CTGF protein ex-pression. Results Combination group could repress patho-proceeding of myocardial fibrosis efficiently, im-prove the activity of CAT and GSH-Px in myocardium and lower the concentration of AGEs in serum, as well as reduce the expression of AGEs and CTGF in myo-cardium. Conclusions Ganoderma lucidum polysac-charides and metformin could prevent myocardial fibro-sis. The possible mechanism may be related to repress-ing oxidative stress of myocardium, lowering serum AGEs and down regulating AGEs and CTGF of myocar-dium.