CAJ | 학술논문

测定大鼠血浆中含丁香苦苷的量，并对具有抗乙肝病毒的丁香苦苷单体和丁香苦苷PLGA纳米粒进行大鼠体内药动学比较研究．采用色谱柱：Diamonsil C18（5μm，250×4．6 mm）流动相为甲醇-水（50∶50，V/V）；流速为1．0 mL/min；检测波长为221 nm；进样量为20μL．测定Wistar大鼠尾静脉注射丁香苦苷后不同时间血浆中的药物质量浓度．给药剂量相同（10 mg/mL ）时，丁香苦苷PLGA溶液的T1/2α和T1/2β值是丁香苦苷溶液的1．23倍和2．25倍．AUC值是丁香苦苷溶液的3．09倍，血浆清除率约为丁香苦苷溶液的1/3．经过数据拟合发现丁香苦苷单体与丁香苦苷PLGA纳米粒在大鼠体内过程均符合二室模型，载药纳米粒给药后，使丁香苦苷能在体内较长时间维持较高的血药质量浓度，起到一定的缓释作用．
측정대서혈장중함정향고감적량，병대구유항을간병독적정향고감단체화정향고감PLGA납미립진행대서체내약동학비교연구．채용색보주：Diamonsil C18（5μm，250×4．6 mm）류동상위갑순-수（50∶50，V/V）；류속위1．0 mL/min；검측파장위221 nm；진양량위20μL．측정Wistar대서미정맥주사정향고감후불동시간혈장중적약물질량농도．급약제량상동（10 mg/mL ）시，정향고감PLGA용액적T1/2α화T1/2β치시정향고감용액적1．23배화2．25배．AUC치시정향고감용액적3．09배，혈장청제솔약위정향고감용액적1/3．경과수거의합발현정향고감단체여정향고감PLGA납미립재대서체내과정균부합이실모형，재약납미립급약후，사정향고감능재체내교장시간유지교고적혈약질량농도，기도일정적완석작용．
This paper determined the content of syringopicroside in the plasma of rats and to make a comparative study on pharmacokinetics of syringopicroside monomer with anti -HBV function and syringopicroside PLGA -nanoparticles in the vivo of rats .Chromatographic col-umn:Diamonsil C18(5 μm,250 ×4.6 mm)mobile phase: methanol-water(50∶50,V/V);flow rate:1.0 mL/min;detected wavelength:221 nm;sample amount:20 μL.The concen-tration of syringopicroside in the plasma of Wistar rats was measured at different time points after intravenous injection .The T1/2αand T1/2βvalues of syringopicroside polylactic -co-gly-colic acid-nanoparticles were 2.25 times and 1.23 times of that of the syringopicroside so-lution respectively with the same dose ( 10 mg/mL ) .The AUC value of syringopicroside polylactic-co-glycolic acid-nanoparticles was 3.09 times of that of syringopicroside solu-tion and the plasma clearance rate of syringopicroside polylactic -co-glycolic acid-nanop-articles was one third of that of syringopicroside .Through the data fitting method , it was found that both syringopicroside monomer and syringopicroside polylactic -co-glycolic acid-nanoparticles in rats conformed to the two -compartment model .The SYR-PLGA-NP changed the feature of pharmacokinetics in the body of rats by making the syringopicroside remain relatively high concentration in plasma for a longer period ,having a slow -releasing effect.